中国肿瘤生物治疗杂志2025，Vol.32Issue(4)：386-391,6.DOI:10.3872/j.issn.1007-385x.2025.04.006
环状RNA hsa_circ_0046701在胶质瘤组织中的表达及其对胶质瘤U251细胞增殖、迁移与侵袭的影响
The expression of circular RNA hsa_circ_0046701 in glioma tissues and its effect on the proliferation,migration and invasion of glioma U251 cells
摘要
Abstract
Objective:To investigate the expression of circular RNA hsa_circ_0046701 in glioma tissues and its effect on the proliferation,migration and invasion of glioma U251 cells.Methods:Tumor tissue specimens and clinical data of fifty-two glioma patients treated surgically in Putuo People's Hospital Affiliated to Tongji University between June 2022 and March 2023 were collected,and another 30 normal brain tissue specimens were collected as controls.The expression level of hsa_circ_0046701 in glioma tissues was detected by qPCR to analyze the relationship between the level and clinical characteristics of the patient.Kaplan-Meier method was used to analyze the relationship between hsa_circ_0046701 level and survival prognosis.Using RNA interference technology,circ_0046701 overexpression and empty vector,siRNA-circ_0046701 and negative control(si-NC)plasmid were transfected into glioma U251 cells,respectively.The experiment cells were divided into the circ 0046701 OE group,Vector group,si-circ_0046701 group and si-NC group.CCK-8 method and Transwell assay were applied to detect the proliferation,migration and invasion abilities of cells in each group.Western blotting was applied to detect the expressions of vimentin,Snail,E-cadherin and cyclin D1 proteins in cells of each group.Results:The expression level of hsa_circ_0046701 in glioma tissues was significantly higher than that in normal brain tissues(P<0.01).The percentage of patients with WHO glioma grading(grades Ⅲ～Ⅳ)in the hsa_circ_0046701 high-expression group was significantly higher than that of the hsa_circ_0046701 low expression group(P<0.01).The OS of patients in the high-expression group was significantly shorter than that of the low-expression group.Compared with those in the si-NC group,the proliferation ability of U251 cells in the si-circ_0046701 group was significantly reduced(P<0.05 or P<0.01),and the numbers of migrated and invaded cells decreased significantly(both P<0.01).The expressions of vimentin,Snail,and cyclin D1 proteins in the cells was significantly reduced(all P<0.01),while the expression of E-cadherin protein increased significantly(P<0.01).Compared with those in the Vector group,the proliferation ability of U251cells in the circ_0046701 OE group increased significantly(P<0.01);the numbers of migrated and invaded cells increased significantly(both P<0.01);the expressions of vimentin,Snail,and cyclin D1 proteins in the cells increaseds significantly(all P<0.01),and the expression of E-cadherin protein decreased significantly(P<0.01).Conclusion:Circular RNA hsa_circ_0046701 is highly expressed in glioma tissues,and is correlated with poor patient prognosis.Knockdown of hsa_circ_0046701 expression could inhibit the proliferation,migration and invasion of glioma U251cells.关键词
环状RNA/hsa_circ_0046701/胶质瘤/U251细胞/增殖/迁移/侵袭Key words
circular RNA(circRNA)/hsa_circ_0046701/glioma/U251 cell/proliferation/migration/invasion分类
临床医学引用本文复制引用
曹崇威,王松涛,王明磊,刘应许..环状RNA hsa_circ_0046701在胶质瘤组织中的表达及其对胶质瘤U251细胞增殖、迁移与侵袭的影响[J].中国肿瘤生物治疗杂志,2025,32(4):386-391,6.基金项目
上海市普陀区人民医院院级科研项目(No.2023rmky04) （No.2023rmky04）
