中国肿瘤生物治疗杂志2025，Vol.32Issue(4)：392-397,6.DOI:10.3872/j.issn.1007-385x.2025.04.007
健脾复胃颗粒通过IL-6/JAK/STAT3轴干预胃癌前病变黏膜损伤及其机制
The intervention of Jianpifuwei granule on mucosal injury of precancerous lesion of gastric cancer via the IL-6/JAK/STAT3 axis and its mechanisms
摘要
Abstract
Objective:To explore the intervention effect of Jianpifuwei granule(JPFWG)on gastric mucosal injury in rats with precancerous lesions of gastric cancer(PLGC)by regulating the IL-6/JAK/STAT3 signaling pathway and its mechanism.Methods:The PLGC model rats were established by MNNG combined with composite factors,and randomly divided into 6 groups(20 rats/group):the blank group(untreated),model group(treated with normal saline),Weimeisu group(0.05 g/mL Weimeisu),JPFWG low-dose group(JPFWG-L,0.088 g/mL),JPFWG medium-dose group(JPFWG-M,0.176 g/mL)and JPFWG high-dose group(JPFWG-H,0.351 g/mL).After being treated with Weimeisu and different doses of JPFWG for 12 weeks,the animals were anesthetized and sacrificed,and the gastric tissues were collected.The pathological changes of gastric mucosa were observed by H-E staining,and the expression levels of IL-6-mediated JAK/STAT3 signaling pathway factors(including IL-6,JAK and STAT3)and the mRNA and proteins of their downstream target genes c-Myc and cyclin D1 in gastric mucosal tissues were detected by immunohistochemistry,qPCR and WB.Results:Compared with that in the model group,the gastric mucosal inflammatory cell infiltration was reduced in the Weimeisu group and the low,medium,and high dose groups of JPFWG;pathological condition was improved,most obviously in the high dose group of JPFWG;The protein expressions of IL-6,JAK1 and STAT3 decreased significantly(P<0.05 or P<0.01).The expressions of mRNA and proteins of c-Myc and cyclin D1 in the gastric mucosal tissues of the rats in the Weimeisu group and JPFWG-H group decreased significantly(P<0.05 or P<0.01).Conclusion:JPFWG can improve the histopathological changes of gastric mucosa in rats with PLGC,and its mechanism may be down-regulating the expressions of c-Myc and cyclin D1 through regulating IL-6/JAK/STAT3 signal pathway,thus blocking inflammation-cancer transformation process.关键词
健脾复胃颗粒/胃癌癌前病变/IL-6/JAK/STAT3信号通路Key words
Jianpifuwei granule(JPFWG)/precancerous lesions of gastric cancer(PLGC)/IL-6/JAK/STAT3 signal pathway分类
临床医学引用本文复制引用
康菊英,关慧颖,李培润,张立明,段永强..健脾复胃颗粒通过IL-6/JAK/STAT3轴干预胃癌前病变黏膜损伤及其机制[J].中国肿瘤生物治疗杂志,2025,32(4):392-397,6.基金项目
宁夏回族自治区科技厅自然基金(No.2021AAC03410) （No.2021AAC03410）
宁夏卫健委课题(No.2022-NWKY-038) （No.2022-NWKY-038）
