中国肿瘤生物治疗杂志2025，Vol.32Issue(4)：398-404,7.DOI:10.3872/j.issn.1007-385x.2025.04.008
lncRNA EBLN3P调节miR-369-3p/CCND1轴对甲状腺癌B-CPAP细胞增殖、迁移和EMT进程的影响
The effects of lncRNA EBLN3P on the proliferation,migration and epithelial-mesenchymal transition(EMT)of thyroid cancer B-CPAP cells by regulating the miR-369-3p/CCND1 axis
摘要
Abstract
Objective:To investigate the effects of long non-coding RNA(lncRNA)endogenous Bornavirus-like nucleoprotein 3 pseudogene(EBLN3P)on the proliferation,migration and epithelial-mesenchymal transition(EMT)of thyroid cancer B-CPAP cells by regulating the miR-369-3p/CCND1 axis.Methods:20 samples of thyroid cancer and corresponding adjacent tissue specimens surgically removed at Hainan Cancer Hospital between May 2020 and May 2021,as well as thyroid cancer B-CPAP cells were collected.The levels of EBLN3P,miR-369-3p and CCND1 mRNA in cancer tissues and cells were detected using qPCR and Western blot(WB)methods.The dual-luciferase reporter gene assay was used to validate the targeting relationship among lncRNA EBLN3P,CCND1 and miR-369-3p.B-CPAP cells were randomly divided into the control group,sh-NC group,sh-EBLN3P group,sh-EBLN3P+anti-NC group and sh-EBLN3P+anti-miR-369-3p group.The colony formation assay was used to detect the number of colony formation in each group.The scratch wound healing assay and Transwell assay were performed to evaluate cell migration ability.WB was used to detect the expression of EMT-related proteins.The nude mouse xenograft model of B-CPAP cells was constructed to observe the effect of silencing EBLN3P on the growth of xenograft tumors.Results:The expressions of lncRNA EBLN3P and CCND1 mRNA were up-regulated,and the expression of miR-369-3p was down-regulated in thyroid cancer tissues and B-CPAP cells(all P<0.05).lncRNA EBLN3P had binding sites with miR-369-3p,and CCND1 had binding sites with miR-369-3p,and there was a targeting relationship.Compared with those in the sh-NC group,the number of clone formation,the scratch healing rate and the number of cell migration in the sh-EBLN3P group decreased(all P<0.05);the expressions of EBLN3P,CCND1,Ki67,MMP-2,N-cadherin and vimentin was down-regulated;the expressions of miR-369-3p and E-cadherin was up-regulated(all P<0.05).Compared with those in the sh-EBLN3P+anti-NC group,the expression of miR-369-3p in the sh-EBLN3P+anti-miR-369-3p group was down-regulated;the number of clone formation,scratch healing rate and the number of cell migration increased(all P<0.05);the expressions of CCND1,Ki67,MMP-2,N-cadherin and vimentin was up-regulated;the expression of E-cadherin was down-regulated(all P<0.05).Compared with those in the sh-NC group,the volume and weight of B-CPAP cell nude mouse xenograft tumors in the sh-EBLN3P group were significantly reduced(both P<0.05).Conclusion:The expression of lncRNA EBLN3P is up-regulated in thyroid cancer cells and tissues.Silencing EBLN3P can inhibit the proliferation,migration and EMT of thyroid cancer B-CPAP cells by targeting and regulating the miR-369-3p/CCND1 axis.关键词
甲状腺癌/B-CPAP细胞/长链非编码RNA/内源性博尔纳病毒样核蛋白3假基因/miR-369-3p/细胞周期蛋白D1/增殖/迁移/上皮间质转化Key words
thyroid cancer/B-CPAP cell/long non-coding RNA(lncRNA)/endogenous Bornavirus-like nucleoprotein 3 pseudogene(EBLN3P)/miR-369-3p/cyclin D1(CCND1)/proliferation/migration/epithelial-mesenchymal transition(EMT)分类
临床医学引用本文复制引用
赵方腾,孙琪,钱永..lncRNA EBLN3P调节miR-369-3p/CCND1轴对甲状腺癌B-CPAP细胞增殖、迁移和EMT进程的影响[J].中国肿瘤生物治疗杂志,2025,32(4):398-404,7.基金项目
海南省自然科学基金高层次人才项目(No.821RC723) （No.821RC723）
