中兽医医药杂志2025，Vol.44Issue(3)：19-25,7.DOI:10.13823/j.cnki.jtcvm.2025.018
沙门菌噬菌体P6的抑菌作用评价
Evaluation of bacteriostatic activity of Salmonella phage P6
摘要
Abstract
This study aimed to investigate the inhibitory effect of short-tailed phage P6 on multi-drug resistant Salmonella,so as to provide experimental basis for the development of novel antimicrobial strategies.In this experiment,the inhibitory effects of P6 on 43 multi-drug resistant isolates of S.typhimurium and S.enteritidis in floating state and biofilm state were systematically evaluated by double-agar plate method and 96-well plate crystal violet staining method.The results showed that after using the double-layer agar plate method to inhibit 43 strains of multidrug-resistant Salmonella with phage P6,phage P6 could lyse 51.2%of the floating drug-resistant Salmonella strains,and the lysate rate of S.enteritidis was 40.0%(4/10)and that of S.typhimurium was 54.5%(18/33).The most common resistance profile among the 43 Salmonella strains was PG-AMX-AMP-STR(44.2%),and the lytic activity of phage P6 against Salmonella strains with this resistance profile reached 93.8%(15/16).At the same time,the lytic phage P6 could inhibit the biofilm of different multi-drug resistant Salmonella by degrading extracellular polymeric substances,and the phage P6 could reduce the biofilm by 11.2%-88.6%within 3 hours.The highest biofilm reduction was achieved in 78.4%of resistant S.enteritidis and 88.6%of resistant S.typhimurium.The average biofilm reduction rate of P6 against resistant S.typhimurium was 45.2%,and the average biofilm reduction rate against resistant S.enteritidis was 64.4%.This experiment proved that P6 had certain inhibitory effects on the floating and biofilm states of the two serotypes of multidrug-resistant Salmonella,which could provide important data support for the optimization and promotion of phage therapy,and also lay the foundation for the combined application of phage with other antibacterial agents.关键词
鼠伤寒沙门菌/肠炎沙门菌/浮游态/生物膜态/耐药性/噬菌体/抑制作用Key words
S.typhimurium/S.enteritidis/floating state/biofilm state/drug resistance/phage/inhibition分类
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吴际坤,魏书琦,俞宁,周鲁阳,俞长旭,王傲菲,杨硕,宋法慧,朱岩丽..沙门菌噬菌体P6的抑菌作用评价[J].中兽医医药杂志,2025,44(3):19-25,7.基金项目
山东省重点研发计划项目(2022CXGC010606) （2022CXGC010606）
