中兽医医药杂志2025，Vol.44Issue(3)：33-39,7.DOI:10.13823/j.cnki.jtcvm.2025.025
黄芩总黄酮的超声提取工艺及体外抗氧化活性研究
Optimization of ultrasonic extraction process and antioxidant activity evaluation of total flavonoids from Scutellaria baicalensis
摘要
Abstract
Single-factor experiments integrated with response surface methodology(RSM)were carried out,using flavonoid extraction yield as the evaluation criterion,to optimize the ultrasound-assisted extraction process of total flavonoids from Scutellaria baicalensis.A sodium nitrite-aluminum nitrate colorimetric method was employed for quantitative analysis.Key parameters—solid-liquid ratio,ethanol concentration,extraction time,and ultrasonic temperature—were systematically evaluated via single-factor testing.Based on variance analysis,solid-liquid ratio,ethanol concentration,and ultrasonic temperature were selected as critical variables for RSM optimization.Concurrently,in vitro antioxidant activity was assessed through DPPH·and ABTS⁺·radical scavenging assays.Results demonstrated that the optimal parameters were solid-liquid ratio of 1∶20(g∶mL),60%ethanol,and extracting for 50 minutes at 50℃,a total flavonoid yield achieved 8.47%,showing close agreement with model predictions(relative error<2.1%).At a concentration of 800 mg/L,the extract exhibited peak scavenging rates of 88.13%(DPPH·)and 75.13%(ABTS⁺·),confirming significant antioxidant efficacy.This work establishes a robust methodology for efficient flavonoid recovery and provides foundational data for pharmaceutical development.关键词
黄芩/总黄酮/超声辅助提取/响应面试验/抗氧化活性Key words
Scutellaria baicalensis/total flavonoids/ultrasonic-assisted extraction/response surface methodology/antioxidant activity分类
农业科技引用本文复制引用
陶卿,符琪星,张波,莫传丽,潘勤春,黄东..黄芩总黄酮的超声提取工艺及体外抗氧化活性研究[J].中兽医医药杂志,2025,44(3):33-39,7.基金项目
广西工业职业技术学院校级科研项目(GYKY2022003A) （GYKY2022003A）
广西高校中青年教师(科研)基础能力提升项目(2023KY1310) （科研）
