不同水位育苗方式对烤烟幼苗根系和耐冷性的影响OA

In order to explore the effects of different water level seedling cultivation methods on the root system and cold tolerance of flue-cured tobacco seedlings,flue-cured tobacco variety Xiangyan No.7 was used as material,and two seed-ling cultivation methods were set up in shallow water level and deep water level.The seed germination,root phenotype,physiological activity and other indicators of flue-cured tobacco seedlings under different light conditions(0,4 000 Lux)were compared and analyzed.The results showed that compared with the deep water seedling treatment,the germination rate and germination energy of flue-cured tobacco seedlings treated in shallow water seedling treatment increased by 6.25%and 21.88%,respectively,and the root length,root surface area,root tip number and root bifurcation number increased by 1.06 times,16.84%,81.5%and 43.42%,respectively.The root activity after 0,6,12 hours of low temperature treatment increased by12.64%,44.47%and51.87%respectively.Whether under cold stress under suitable light(4 000 Lux)or no light(0 Lux)conditions,the activities of POD,CAT and SOD in shallow water seedling treatment were significantly higher than those in deep water seedling treatment,and both treatments had higher levels of antioxidant enzyme activities under suitable light conditions.The enrichment analysis results of differentially expressed genes in different seedling treat-ments before and after low temperature stress showed that the seedling treatment in shallow water had strong energy conver-sion ability,and more products that responded to cold stress(such as terpene synthase and peptidases)could be produced when responding to cold stress.Comprehensive analysis shows that the shallow water seedling treatment is conducive to cul-tivating more developed deep root systems,enhancing root vitality,increasing antioxidant enzyme activities,and improving the ability of flue-cured tobacco seedlings to cope with cold stress.