植物学报2025，Vol.60Issue(3)：377-392,16.DOI:10.11983/CBB24117
菠菜AT-hook基因家族鉴定与表达谱分析
Identification of the Spinach AT-hook Gene Family and Analysis of Expression Profiles
摘要
Abstract
INTRODUCTION:The AT-hook motif nuclear localized(AHL)gene family is a highly conserved transcription factors in-volved in plant growth,development,and stress responses,but their roles in spinach are still unknown. RATIONALE:To reveal the basic characteristics of the AHL family in spinach,members of the spinach SoAHL family were identified at the whole-genome level,and their physicochemical properties,gene structure,conserved motifs,pro-moter elements,and salicylic acid-responsive expression profiles were analyzed in this study. RESULTS:The results revealed 19 SoAHL family members in the spinach genome,which were unevenly distributed across six chromosomes.These SoAHL members can be classified into three branches,with 10 members in subfamily Ⅰand 9 members in subfamily Ⅱ.The sequence composition of PPC and AT-hook conserved motifs varies among subfami-lies;most of the SoAHL genes are located in the nucleus,cytoplasm,and mitochondrion.Members of subfamily Ⅰ of SoAHL have no introns,whereas members of subfamily Ⅱ contain 4-5 introns.The varying numbers of cis-acting ele-ments relate to phytohormones and abiotic stress responses were distributed upstream of the promoters of the SoAHL members.The SoAHL genes can be expressed in roots,leaves,and petioles,with most genes expressed at relatively high levels in roots.The expression of two SoAHL genes(SOV6g041850.1 and SOV2g038950.1)was significantly in-duced by salicylic acid treatment.The expression profiles and salicylic acid-induced expression levels of SOV2g031340.1 and SOV4g018880.1 were highly correlated with the folic acid content,which may play a role in the spinach response to the salicylic acid signaling pathway.The transient overexpression of SOV4g018880.1 increased the folate content of spinach leaves by 1.75 times. CONCLUSION:The results from the sequence characteristics,expression profiles and exogenous salicylic acid treatment revealed that the SoAHLs had potential functional diversity and that specific members may have positive effects on spinach folate accumulation.Our results will lay the foundation for further resolving the function of spinach AT-hook genes.关键词
菠菜/AT-hook基因家族/生物信息学/水杨酸Key words
spinach/AT-hook gene family/bioinformatics/salicylic acid引用本文复制引用
杨莉,曲茜彤,陈子航,邹婷婷,王全华,王小丽..菠菜AT-hook基因家族鉴定与表达谱分析[J].植物学报,2025,60(3):377-392,16.基金项目
上海市科技兴农项目(No.2022-02-08-00-12-F01116) （No.2022-02-08-00-12-F01116）
