植物遗传资源学报2025，Vol.26Issue(5)：905-915,11.DOI:10.13430/j.cnki.jpgr.20240909002
基于ISSR分子标记的新疆西天山栒子属种质资源遗传多样性分析
Genetic Diversity Analysis of Cotoneaster Germplasm Resources in Western Tianshan Mountains of Xinjiang Based on ISSR Molecular Markers
摘要
Abstract
To explore the genetic diversity and relationships among the populations of genus Cotoneaster in the Western Tianshan Mountains of Xinjiang municipality,China,we analyzed the genetic diversity of 112 Cotoneaster plants from 15 wild populations using ISSR molecular markers.A total of 166 bands were amplified using 15 ISSR primers,of which.143 were polymorphic,yielding an average polymorphic ratio of 86.29％.The Nei's gene diversity index(H)and Shannon's information index(I)were 0.2124 and 0.3254,respectively.The genetic differentiation coefficient(Fst)among populations was 0.4259,and the gene flow(Nm)was 0.6740.These results indicated significant genetic differentiation among the wild populations of Cotoneaster,with a certain degree of gene exchange occurring between populations.Overall,Cotoneaster populations in the Western Tianshan Mountains of Xinjiang exhibited clear genetic differences and high levels of genetic diversity.At the population level,the Beishan Maoliugou population in Tekes County represented the highest genetic diversity.Clustering results revealed that most populations could be clustered according to their geographical locations,although some geographically close populations did not clustered together.These fingdings can provide an important reference for the effective protection and development and utilization of Cotoneaster germplasm resources in the Western Tianshan Mountains of Xinjiang municipality,China.关键词
新疆西天山/栒子属/遗传多样性/ISSR分子标记Key words
Western Tianshan of Xinjiang/Cotoneaster/genetic diversity/ISSR molecular markers引用本文复制引用
阿克居力得孜·努尔改里得,李茹雪,杨禧雨,陆彪,周龙..基于ISSR分子标记的新疆西天山栒子属种质资源遗传多样性分析[J].植物遗传资源学报,2025,26(5):905-915,11.基金项目
2022新疆维吾尔自治区高校基本科研业务费项目(XJEDU2022Z004) （XJEDU2022Z004）
第三次新疆综合科学考察课题(2021xjkk0501)2022 Basic Scientific Research Business Fee Project of Colleges and Universities in Xinjiang Uygur Autonomous Region(XJEDU2022Z004) （2021xjkk0501）
The Third Comprehensive Scientific Expedition in Xinjiang(2021xjkk0501) （2021xjkk0501）
