植物研究2025，Vol.45Issue(3)：406-418,13.DOI:10.7525/j.issn.1673-5102.2025.03.011
玉米GLP家族基因鉴定及其响应丛枝菌根共生表达
Identification of the Maize GLP Family Genes and Their Expression in Response to Arbuscular Mycorrhizal Symbiosis
摘要
Abstract
Germin-like proteins(GLPs)are a class of highly conserved stress-responsive proteins that can specifically respond to symbiotic interactions.This study,based on the whole-genome information of maize B73,used bioinformatics approaches to identify and analyze the characteristics of the ZmGLP gene family.The expression patterns of ZmGLP genes during arbuscular mycorrhizal fungi(AMF)symbiosis were examined by using transcriptome data.This study identified a total of 45 ZmGLP genes,which were distributed across nine chromosomes,with 25 of the genes being tandem duplications.By analysis of a phylogenetic tree,ZmGLP genes were classified into five distinct subfamilies.Notably,there were significant differences in tissue expression patterns among different ZmGLP genes.Promoter cis-element analysis showed that ZmGLP promoters contained elements responsive to light,stress,and growth and development.Interestingly,the promoter of and ZmGLP6-1 were significantly upregulated in expression during the late stage of symbiosis.These genes were located in different evolutionary branches compared to the reported symbiosis-related GLP genes,suggesting that these genes may be involved in functions related to the late stage of mycorrhizal symbiosis.Functional studies of ZmGLP3-3 showed that,compared to wild-type maize plants,the mycorrhizal symbiosis rate of the mutant zmglp3-3 was significantly reduced.In summary,this study provided a comprehensive theoretical basis for the exploration of symbiosis-related ZmGLP genes in maize,offering insights into their potential roles and functions in plant-fungal interactions.关键词
丛枝菌根真菌/类萌发素蛋白/基因家族/菌根共生/表达分析Key words
arbuscular mycorrhizal fungi/germin-like proteins/gene family/mycorrhizal symbiosis/expression analysis分类
生物科学引用本文复制引用
春建惠,董文龙,屠元超,刘芳,徐云剑..玉米GLP家族基因鉴定及其响应丛枝菌根共生表达[J].植物研究,2025,45(3):406-418,13.基金项目
云南省科技计划项目(202401BF070001-002 （）
202301AT070106) （）
云南省教育厅科学研究基金项目(KC-24248854). （KC-24248854）
