植物研究2025，Vol.45Issue(3)：433-446,14.DOI:10.7525/j.issn.1673-5102.2025.03.013
不同植物来源内生菌提取物抗氧化、抗紫外及抑菌功能评价
Evaluation of Antioxidant,Anti-ultraviolet Radiation,and Antibacterial Functions of Endophytic Extracts from Different Plant Sources
摘要
Abstract
This study evaluated the antioxidant,antimicrobial,UV radiation resistance properties,and secondary metabolite content of fermented extracts from 20 endophytic fungi of diverse plant origins to assess their potential in cosmetic applications.ABTS and DPPH radical scavenging rates of fermentation broths and mycelial extracts were measured using microplate reader colorimetry,while ferric ion reduction capacity was determined via FRAP assay.Antimicrobial activity was assessed using the Oxford cup method.The results indicated strong antioxidant activity in fermentation broths of strains XY1,YZ6,ZJ29,XG4-2,and ZBS49.ZJ29 exhibited the highest DPPH radical scavenging rate(93.62%),while XY1 showed the highest ABTS radical scavenging rate(90.47%).ZJ29 fermentation broth also demonstrated an ABTS radical scavenging rate>50%and the highest FRAP value(2 974.55 µmol·L-¹).Tyrosinase inhibition by ZJ29 and ZL18-1 mycelial extracts reached 60.62%and 98.10%,respectively,with ZJ29 fermentation broth showing the strongest inhibition(69.78%).ZJ29 fermentation broth exhibited the highest inhibition against Staphylococcus aureus.The UVA and UVB absorbance of YZ6 and ZJ29 was higher than 2.8,and the UVA absorbance of YZ6 and ZJ29 was higher than 2.0 and 1.5,respectively.YZ6 mycelial extract also showed strong resistance to UVB and UVA,with absorbance of 1.5-2.5.YH3 and XY1 mycelial extracts contained the higher polyphenol(18.47 and 15.36 mg·g-¹)and flavonoid(4.35 and 7.15 mg·g-¹)content,respectively.ZL23 and ZJ29 had the higher triterpene content(16.79 and 11.20 mg·g-¹,respectively).In conclusion,ZJ29 mycelial extracts had superior antimicrobial,UV radiation resistance,and antioxidant activities,followed by YZ6 and XY1,highlighting their significant potential for daily chemical products.关键词
内生真菌/次生代谢产物/抗氧化/抗紫外线辐射/抑菌Key words
endophytic fungi/secondary metabolites/antioxidation/anti-ultraviolet radiation/bacteriostasis分类
生物科学引用本文复制引用
邹坤林,姜厶源,魏亚静,张顺,李鹏,尹静..不同植物来源内生菌提取物抗氧化、抗紫外及抑菌功能评价[J].植物研究,2025,45(3):433-446,14.基金项目
黑龙江省自然科学基金项目(ZD2023C004) （ZD2023C004）
东北林业大学本科创新项目(202310225164) （202310225164）
黑龙江省省属科研院所基本科研业务费项目(ZNBZ2023GJS06) （ZNBZ2023GJS06）
东北林业大学本科教育教学改革项目(DGYGJ2022-02). （DGYGJ2022-02）
