植物研究2025，Vol.45Issue(3)：447-459,13.DOI:10.7525/j.issn.1673-5102.2025.03.014
不同菌根类型树种与桉树混交对土壤微生物群落的影响
The Effects of Mixed Eucalyptus Plantations with Different Mycorrhizal Tree Species on Soil Microbial Community
摘要
Abstract
Mixed-species plantations can promote soil nutrient cycling,productivity,and ecosystem functions.Mycorrhizal associations play important roles in affecting species coexistence and nutrient cycling in mixed plantations.However,our understanding on the effects of mixed tree species with different mycorrhizal types on soil microbial community associated with soil nutrients of Eucalyptus plantations remains limited.In this study,we conducted metagenomic sequencing to investigate the changes in soil microbial community structure and functions,co-occurrence networks between bacteria and fungi,and their relationships with soil nutrients for one monospecific Eucalyptus plantation and three mixed Eucalyptus plantations with different mycorrhizal tree species,including Dalbergia odorifera(arbuscular mycorrhizal and N2-fixing,AM-NF),Michelia macclurei(arbuscular mycorrhizal,AM),and Quercus acutissima(ectomycorrhizal,ECM).The results indicated that the mixed stands significantly increased the richness of soil microbial community,enhanced the abundance of genes related to soil nutrient cycling,and increased the complexity of microbial co-occurrence networks.The mixed plantations of Eucalyptus with D.odorifera had the highest soil microbial richness,relative abundance of bacterial community associated with aerobic chemoheterotrophy and fermentation,and the modularity of fungal-bacterial co-occurrence network;meanwhile,either the bacterial or fungal communities were significantly different from the other three plantations.The mixed Eucalyptus plantations with M.macclurei or Q.acutissima species had the highest abundance of microbial functional genes such as hydrocarbon degradation,nitrogen fixation,and methanotrophy.In addition,the microbial co-occurrence networks in the mixed Eucalyptus plantations with Q.acutissima have the highest number of edges,average degree,and network stability.The richness of key microbes in co-occurrence networks was closely correlated with soil NO-3 level.However,the richness of microbial community of the networks in the mixed Eucalyptus plantations with D.odorifera and M.macclurei was largely associated with soil NH+4 level.关键词
桉树/混交林/菌根类型/土壤微生物/共现网络Key words
Eucalyptus/mixed-species plantations/mycorrhizal type/soil microbial community/co-occurrence network分类
生物科学引用本文复制引用
王亚鑫,朱媛,孟森,明安刚,贾宏炎,覃方锉,陆俊锟..不同菌根类型树种与桉树混交对土壤微生物群落的影响[J].植物研究,2025,45(3):447-459,13.基金项目
中国林业科学研究院基本科研业务费项目(CAFYBB2023MA007) （CAFYBB2023MA007）
国家自然科学基金面上项目(3247141238) （3247141238）
国家重点研发计划课题(2023YFD2201005) （2023YFD2201005）
广东省基础与应用基础研究基金项目(2022A1515010413). （2022A1515010413）
