中药新药与临床药理2025，Vol.36Issue(5)：666-673,8.DOI:10.19378/j.issn.1003-9783.2025.05.002
三黄凝胶对痤疮模型大鼠MyD88/IRAK1/TRAF6/NF-κB信号通路及下游相关因子的影响
Effects of Sanhuang Gel on the MyD88/IRAK1/TRAF6/NF-κB Signaling Pathway and Downstream-Related Factors in Acne Model Rats
摘要
Abstract
Objective To investigate the effects of Sanhuang Gel on the myeloid differentiation factor 88(MyD88)/interleukin-1 receptor-associated kinase 1(IRAK1)/tumor necrosis factor receptor-associated factor 6(TRAF6)/nuclear factor-κB(NF-κB)signaling pathway and the expression of downstream factors in acne model rats,and to explore its mechanism in treating acne.Methods Sixty male Wistar rats were randomly divided into a blank group,a model group,a positive control group(Clindamycin Hydrochloride Gel,10 g·kg-1),and high-,medium-,and low-dose Sanhuang Gel groups(crude drug doses of 280,140,and 70 g·kg-1,respectively),with 10 rats in each group.A composite acne model was induced by applying oleic acid and injecting Propionibacterium acnes at the opening of the right ear auricle,with a lesion area of 1 cm2.After successful model establishment,the treatment groups were topically administered 0.25 g of the corresponding drug once daily for 28 days.Changes in auricular lesions were observed.Pathological changes in auricular tissues were examined using HE staining.RT-qPCR was used to detect the mRNA expressions of MyD88,TRAF6,and NF-κB in auricular tissues.Western Blot was used to measure the protein expression levels of MyD88,IRAK1,TRAF6,NF-κB p65,interleukin-1β(IL-1β),and interleukin-18(IL-18)in auricular tissues.Immunohistochemistry was used to detect the protein expression levels of NOD-like receptor protein 3(NLRP3),cysteine-dependent aspartate-specific protease 1(Caspase-1),and apoptosis-associated speck-like protein containing a CARD(ASC)in auricular tissues.Results Compared with the blank group,the model group showed significant papules,pustules,and cysts on the auricular skin,accompanied by thickening of the stratum corneum and epidermis,massive inflammatory cell infiltration,and a large amount of keratin in hair follicles.The mRNA expression levels of MyD88,TRAF6,and NF-κB p65 in auricular tissues were significantly increased(P＜0.01),and the protein expression levels of MyD88,IRAK1,TRAF6,NF-κB p65,IL-1β,IL-18,NLRP3,ASC,and Caspase-1 were significantly elevated(P＜0.01).Compared with the model group,all treatment groups improved papules,pustules,thickening of the stratum corneum,and inflammatory cell infiltration in the auricular tissues,with the high-dose group showing the best effect.The mRNA expression levels of MyD88,TRAF6,and NF-κB p65 in auricular tissues were significantly reduced in all treatment groups(P＜0.01),and the protein expression levels of MyD88,IRAK1,IL-1β,IL-18,and Caspase-1 were significantly decreased(P＜0.05,P＜0.01).The protein expression levels of TRAF6,NF-κB p65,and ASC in auricular tissues were significantly reduced in the positive control group and high-dose group(P＜0.01).The expression of NLRP3 in auricular tissues was significantly reduced in the positive control group and medium-and high-dose Sanhuang Gel groups(P＜0.05,P＜0.01).Conclusion Sanhuang Gel can significantly improve papules,pustules,and other mild to moderate acne lesions in the auricular tissues of acne model rats,alleviating inflammatory damage.Its mechanism may be related to regulating the MyD88/IRAK1/TRAF6/NF-κB signaling pathway and downstream NLRP3 inflammasome,IL-1β,and IL-18 expression,thereby improving skin immune-inflammatory responses.关键词
三黄凝胶/痤疮/MyD88/IRAK1/TRAF6/NF-κB信号通路/NLRP3炎性小体/炎症因子/大鼠Key words
Sanhuang Gel/acne/MyD88/IRAK1/TRAF6/NF-κB signaling pathway/NLRP3 inflammasome/in-flammatory factors/rats分类
医药卫生引用本文复制引用
杜雪洋,董小平,牛凡琪,郭斐斐,杨鹏斐,金彩云,王思农..三黄凝胶对痤疮模型大鼠MyD88/IRAK1/TRAF6/NF-κB信号通路及下游相关因子的影响[J].中药新药与临床药理,2025,36(5):666-673,8.基金项目
甘肃省高等学校科技成果转化项目(2017D-17) （2017D-17）
甘肃省教育厅科技创新项目(2023A-079) （2023A-079）
甘肃省高校产业支撑计划项目(2025CYZC-046). （2025CYZC-046）
