中药新药与临床药理2025，Vol.36Issue(5)：683-691,9.DOI:10.19378/j.issn.1003-9783.2025.05.004
桃核承气汤通过保护血管内皮预防小鼠脓毒症凝血功能障碍
Taohong Chengqi Decoction Prevents Coagulation Dysfunction in Sepsis by Protecting Vascular Endothelium in Mice
摘要
Abstract
Objective To investigate the effect and mechanism of Taohe Chengqi Decoction(THCQD)on coagulation dysfunction in sepsis.Methods Sixty KM mice were randomly divided into six groups based on body mass:normal group,model group,low-dose THCQD group,high-dose THCQD group,positive control Heparin Sodium Injection group,and Xuebijing Injection group.Except for the normal group,sepsis models were established in the other groups by intraperitoneal injection of carrageenan(Car)and lipopolysaccharide(LPS).The relative length of tail thrombosis and the relative congestion area of auricular capillaries were measured.Activated partial thromboplastin time(APTT)and prothrombin time(PT)were detected using reagent kits.Serum levels of tissue factor(TF),C-reactive protein(CRP),and interleukin-6(IL-6)were measured by ELISA.Pathological changes in the tail,liver,and lung tissues were observed using hematoxylin-eosin(HE)staining.Immunohistochemistry was used to detect the protein expression of von Willebrand factor(vWF)in tail thrombi and vascular endothelial cadherin(VE-Cadherin)in lung tissues.Levels of monocyte chemoattractant protein-1(MCP-1),vascular cell adhesion molecule-1(VCAM-1),and Syndecan-1 in plasma were measured using flow cytometry.An endothelial injury model was established by stimulating EA.hy926 cells with LPS,and RT-PCR was used to detect mRNA levels of interleukin-1β(IL-1β),IL-6,and TF in EA.hy926 cells.Results Compared with the model group,THCQD significantly reduced the auricular congestion area(P＜0.01)and shortened the relative length of tail thrombosis(P＜0.01)at 6 hours post-modeling,shortened APTT and PT(P＜0.01)at 12 hours post-modeling,decreased serum TF(P＜0.01)and IL-6 levels(P＜0.05),reduced plasma levels of MCP-1,VCAM-1,and Syndecan-1(P＜0.05,P＜0.01),lowered peripheral white blood cell counts(P＜0.01),decreased thrombus area in tail veins(P＜0.01),reduced intravascular congestion in liver and lung tissues(P＜0.01),and downregulated the protein expression of vWF and VE-Cadherin(P＜0.05,P＜0.01).THCQD-containing serum significantly reduced LPS-induced mRNA levels of IL-6,IL-1β,and TF in EA.hy926 cells(P＜0.05,P＜0.01).Conclusion Taohe Chengqi Decoction prevents coagulation dysfunction in sepsis by protecting vascular endothelium.关键词
桃核承气汤/脓毒症/凝血功能障碍/血管内皮/小鼠Key words
Taohe Chengqi Decoction/sepsis/coagulation dysfunction/vascular endothelium/mice分类
医药卫生引用本文复制引用
欧倩清,林俊粒,曲丽媛,郑玉莹,刘方乐,匡忠生,林宝琴..桃核承气汤通过保护血管内皮预防小鼠脓毒症凝血功能障碍[J].中药新药与临床药理,2025,36(5):683-691,9.基金项目
国家自然科学基金委青年基金项目(82404817) （82404817）
国家自然科学基金青年项目(82104504) （82104504）
广州市科技局基础与应用基础项目之广州市重点实验室建设项目(2024A03J0360) （2024A03J0360）
国家中医药传承创新中心科研专项(2023ZD01). （2023ZD01）
