中药新药与临床药理2025，Vol.36Issue(5)：729-742,14.DOI:10.19378/j.issn.1003-9783.2025.05.008
白背叶楤木乙酸乙酯提取物通过抑制MAPK信号通路干预OVA所致的哮喘小鼠气道炎症
Ethyl Acetate Extract of Aralia chinensis var.nuda Inhibits Airway Inflammation via Suppressing MAPK Signaling Pathway in OVA-Induced Asthmatic Mice
摘要
Abstract
Objective To explore the potential mechanism of ethyl acetate extract of Aralia chinensis var.nuda(EAEAC)in alleviating airway inflammation in asthmatic mice through network pharmacology and experimental validation.Methods An ovalbumin(OVA)-induced asthmatic mouse model was established to evaluate the ameliorative effects of EAEAC on airway inflammation.The intersection of EAEAC active component targets and asthma disease targets was used to construct a"drugs-components-diseases-targets"network.Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)enrichment analyses were performed,and molecular docking was conducted for key active components and core pathway targets.The key pathway was validated in vivo.Results EAEAC significantly improved airway inflammation infiltration,goblet cell hyperplasia,and airway hyperresponsiveness in the lung tissue of asthmatic mice.It down regulated the levels of IgE and OVA-IgE in serum and inflammatory cytokines(IL-4,IL-5,IL-9,IL-13,IL-25,IL-33,and TSLP)in bronchoalveolar lavage fluid(BALF)(P＜0.05,P＜0.01,P＜0.001).Network pharmacology revealed that the KEGG analysis of EAEAC intervention in asthma was enriched in the MAPK,HIF-1,and PI3K/Akt signaling pathways.Molecular docking results showed that stigmasterol had strong binding affinities with TLR4,MAPK8,and MAPK14.Experimental validation demonstrated that EAEAC reduced the protein expression of MYD88,p-P38/P38,and p-JNK/JNK in the lung tissue of asthmatic mice(P＜0.001,P＜0.01,P＜0.05).Conclusion EAEAC may exert its anti-asthmatic effects by inhibiting the activation of the MAPK pathway,thereby alleviating airway inflammation in asthma.关键词
白背叶楤木乙酸乙酯提取物/哮喘/气道炎症/网络药理学/实验验证/MAPK通路/小鼠Key words
ethyl acetate extract of Aralia chinensis var.nuda/asthma/airway inflammation/network pharmacology/experimental verification/MAPK pathway/mice分类
医药卫生引用本文复制引用
龙玉淮,李媛媛,袁圆,徐双娅,张蝶,陈福长,张进伟,李宝晶..白背叶楤木乙酸乙酯提取物通过抑制MAPK信号通路干预OVA所致的哮喘小鼠气道炎症[J].中药新药与临床药理,2025,36(5):729-742,14.基金项目
国家自然科学基金项目(82260832,81860760) （82260832,81860760）
云南省中医联合专项(202101AZ070001-159) （202101AZ070001-159）
云南省"兴滇英才支持计划"(XDYC-QNRC-2023-0529) （XDYC-QNRC-2023-0529）
生物资源数字化开发应用(202002AA100007) （202002AA100007）
云南省南药可持续利用研究重点实验室项目(202105AG070012). （202105AG070012）
