中药新药与临床药理2025，Vol.36Issue(5)：743-749,7.DOI:10.19378/j.issn.1003-9783.2025.05.009
复元活血汤联合阿托伐他汀对大鼠原位回植皮瓣血管形成及远端供血的影响
Effect of Fuyuan Huoxue Decoction Combined with Atorvastatin on Vascular Formation and Distal Blood Supply in Rat Orthotopic Replanted Skin Flaps
摘要
Abstract
Objective To investigate the effect of Fuyuan Huoxue Decoction combined with Atorvastatin on vascular formation and distal blood supply in rat orthotopic replanted skin flaps.Methods Forty rats were randomly divided into a control group,a Fuyuan Huoxue Decoction group(7.14 g·kg-1),an Atorvastatin group(20 mg·kg-1),and a Fuyuan Huoxue Decoction combined with Atorvastatin group(Fuyuan Huoxue Decoction 7.14 g·kg-1+Atorvastatin Calcium Tablets 20 mg·kg-1),with 10 rats in each group.A dorsal super-long random skin flap model was constructed in each group by surgical method,and a skin flap of 2 cm×8 cm was separated on the rat's back and sutured in situ.The rats in each treatment group were given intragastric administration once a day for 7 consecutive days,while the control group was given the same amount of normal saline.After 7 days,the survival rate of the skin flap tissue was analyzed and calculated;the pathological changes of the skin flap tissue were observed by HE staining;the expression level of CD34 in the skin flap tissue was detected by immunohistochemistry;the levels of interleukin-6(IL-6)and tumor necrosis factor-α(TNF-α)in the skin flap tissue were detected by ELISA;the mRNA and protein expression levels of protein kinase D1(PKD1),histone deacetylase 5(HDAC5),and vascular endothelial growth factor(VEGF)in the skin flap tissue were detected by RT-qPCR and Western Blot.Results Compared with the control group,the survival area of the skin flaps in each treatment group was significantly increased(P＜0.05),and the survival rate of the skin flaps was significantly increased(P＜0.05);the number of neutrophils in the skin flap tissue was significantly reduced(P＜0.05),the expression level of CD34 was significantly increased(P＜0.05),and the levels of IL-6 and TNF-α were significantly decreased(P＜0.05);the mRNA and protein expressions of PKD1,HDAC5,and VEGF in the skin flap tissue were significantly up-regulated(P＜0.05).Compared with the Fuyuan Huoxue Decoction group or the Atorvastatin group,the survival area of the skin flaps in the Fuyuan Huoxue Decoction combined with Atorvastatin group was significantly increased(P＜0.05),and the survival rate of the skin flaps was significantly increased(P＜0.05);the number of neutrophils in the skin flap tissue was significantly reduced(P＜0.05),the expression level of CD34 was significantly increased(P＜0.05),and the levels of IL-6 and TNF-α were significantly decreased(P＜0.05);the mRNA and protein expressions of PKD1,HDAC5,and VEGF in the skin flap tissue were significantly up-regulated(P＜0.05).Conclusion The combination of Fuyuan Huoxue Decoction and Atorvastatin can promote vascular formation,improve microcirculation,and enhance distal blood supply in rat orthotopic replanted skin flaps,with effects superior to those of the single treatment groups,which may be related to the activation of the PKD1/HDAC5/VEGF pathway.关键词
复元活血汤/阿托伐他汀/联合用药/皮瓣原位回植/皮瓣成活率/远端供血/PKD1/HDAC5/VEGF通路/大鼠Key words
Fuyuan Huoxue Decoction/Atorvastatin/combination therapy/orthotopic skin flap replantation/skin flap survival rate/distal blood supply/PKD1/HDAC5/VEGF pathway/rats分类
医药卫生引用本文复制引用
曾静达,陈孝均,杨林,张箭..复元活血汤联合阿托伐他汀对大鼠原位回植皮瓣血管形成及远端供血的影响[J].中药新药与临床药理,2025,36(5):743-749,7.基金项目
四川省科技厅项目(2022JDKP0051) （2022JDKP0051）
泸州市科技计划项目(2019-SYF-40). （2019-SYF-40）
