中国现代中药2025，Vol.27Issue(5)：860-868,9.DOI:10.13313/j.issn.1673-4890.20241018002
远志寡糖酯改善D-半乳糖致小鼠学习记忆障碍的作用
Effect of Oligosaccharide Esters from Polygala tenuifolia Willd.in Ameliorating D-galactose-induced Learning and Memory Impairment in Mice
摘要
Abstract
Objective:To investigate the effect of oligosaccharide esters from Polygala tenuifolia Willd.(OE)on learning and memory impairment in a rapidly-aging dementia mouse model induced by D-galactose.Methods:Eight-week-old Kunming mice were randomly divided into the control group,model group,donepezil group,and OE treatment groups(28.67,57.33 mg·kg-1).Brain aging was induced by intraperitoneal injection of D-galactose,and OE or donepezil was administered orally once daily for 60 consecutive days.Learning and memory abilities were assessed using the step-down test and the Morris water maze test.Serum was collected to measure superoxide dismutase(SOD)activity and malondialdehyde(MDA)levels.Immunohistochemistry was used to detect brain-derived neurotrophic factor(BDNF)expression in the cerebral cortex,while Western blot was performed to analyze the protein levels of protein kinase B(Akt),cAMP response element-binding protein(CREB),and BDNF in the cerebral cortex.Results:Compared with the control group,the model group exhibited significantly prolonged escape latency(P<0.05)and first platform-crossing latency(P<0.01)in the Morris water maze test,along with a significant reduction in platform-crossing frequency(P<0.01).In the step-down test,the first-jump latency was significantly shortened(P<0.01),while the number of errors increased significantly(P<0.01).Additionally,the model group showed significantly decreased serum SOD activity(P<0.01)and increased MDA levels(P<0.01),as well as significantly reduced levels of p-Akt/Akt,p-CREB/CREB,and BDNF in the cerebral cortex(P<0.01).Compared with the model group,OE treatment significantly shortened escape latency(P<0.05)and first platform-crossing latency(P<0.01),while increasing platform-crossing frequency(P<0.01)in the Morris water maze test.In the step-down test,OE prolonged the first-jump latency(P<0.01)and reduced the number of errors(P<0.01).Furthermore,OE enhanced serum SOD activity(P<0.05,P<0.01),reduced MDA levels(P<0.01),and upregulated the expression of p-Akt/Akt,p-CREB/CREB,and BDNF in the cerebral cortex(P<0.05,P<0.01).Conclusion:OE can alleviate learning and memory impairments in D-galactose-induced rapidly aging dementia mice,potentially by reducing oxidative damage and regulating the Akt/CREB/BDNF signaling pathway.关键词
远志寡糖酯/D-半乳糖/学习记忆障碍/抗氧化/蛋白激酶B/环磷腺苷效应元件结合蛋白/脑源性神经营养因子信号通路Key words
oligosaccharide esters from Polygala tenuifolia Willd./D-galactose/learning and memory impairment/antioxidation/Akt/CREB/BDNF signaling pathway分类
中医学引用本文复制引用
邢瀚文,桑旭星,印艳萍,谢岚,姜艳艳,方芳..远志寡糖酯改善D-半乳糖致小鼠学习记忆障碍的作用[J].中国现代中药,2025,27(5):860-868,9.基金项目
国家自然科学基金项目(81373995) （81373995）
北京中医药大学校级科研纵向发展基金项目(2019-ZXFZJJ-106) （2019-ZXFZJJ-106）
