中国现代中药2025，Vol.27Issue(5)：869-877,9.DOI:10.13313/j.issn.1673-4890.20241030002
基于肠道菌群的天竺黄对发热幼鼠模型的退热作用探讨
Antipyretic Effect of Bambusae Concretio Silicea on Fever Model of Juvenile Rats Based on Intestinal Flora
摘要
Abstract
Objective:To study the effect of Bambusae Concretio Silicea(BCS)on the body temperature of juvenile rats with yeast suspension-induced fever and explore its mechanism from the perspective of intestinal flora.Methods:Sixty male SD rats were randomly divided into the following groups:a model group,groups treated with high-dose,medium-dose,and low-dose BCS Decoction(hereinafter referred to as TSG,TSZ,and TSD),groups treated with high-dose,medium-dose,and low-dose BCS Powder(hereinafter referred to as TFG,TFZ,and TFD),a gypsum decoction group(SG),and an ibuprofen group(BLF),with six rats in each group.All groups except for the control group received an injection of 20%yeast suspension(10 mL·kg-1)to establish a fever model.Two hours after modeling,the temperature of the rats increased significantly(>0.6℃),with the temperature difference being statistically significant compared to the control group(P<0.001),indicating successful modeling.The initial rectal temperature was measured,and subsequent measurements were taken at 1,2,3,4,and 5 h after administration.In the groups treated with BCS,one additional administration was given at 2 h and 4 h after the initial treatment.The rats in the control group,model group,and TFG group were euthanized,and the intestinal feces were collected and analyzed to assess intestinal flora.Results:Except for that in the TSD group,the body temperature of the rats in the other seven groups decreased after administration,with statistically significant differences observed in the TFG,SG,and BLF groups between 2 h and 5 h after administration(P<0.01),while no significant differences were observed in the remaining groups.Correlation analysis with intestinal flora showed that at the phylum level,the relative abundance of Desulfobacterota and Actinobacteriota in the TFG group was significantly increased compared with that in the model group(P<0.01).The relative abundance ratio of Firmicutes and Bacteroidetes(F/B)was adjusted to the normal level.At the genus level,compared to the model group,the TFG group showed statistically significant differences in[Eubacterium]_coprostanoligenes_group,Prevotellace_UCG-001,Lactobacillus,Alistipes,and Marvinbryantia(P<0.05),mainly by increasing the relative abundance of[Eubacterium]_coprostanoligenes_group,Lactobacillus,and Alistipes,as well as decreasing the relative abundance of Prevotellace_UCG-001 and Marvinbryantia.The linear discriminant analysis effect size(LEfSe)analysis shows that f_Eubacterium_coprostanoligenes_group and g_Eubacterium_coprostanoligenes_group were the dominant taxa,and the relative abundance of the two microbial genera in the TFG group was significantly higher than that in the other two groups.Moreover,the linear discriminant analysis(LDA)scores of o_Bacteroidales,c_Bacteroidia,and p_Bacteroidota were higher than those of other taxonomic units,indicating that they had a greater effect on the difference between groups.Conclusion:BCS Powder can attenuate the temperature rise of yeast-induced fever in juvenile rats,and its antipyretic effect is relatively mild,with the mechanism of action related to restoring the intestinal flora structure in the fever model of juvenile rats to the healthy state.关键词
天竺黄/幼鼠/干酵母/退热机制/肠道菌群Key words
Bambusae Concretio Silicea/juvenile rat/dried yeast/antipyretic mechanism/intestinal flora分类
中医学引用本文复制引用
齐玉鑫,孔祥河,任广喜,姜丹,刘春生..基于肠道菌群的天竺黄对发热幼鼠模型的退热作用探讨[J].中国现代中药,2025,27(5):869-877,9.基金项目
国家自然科学基金项目(82173921) （82173921）
中央高校横向课题项目(2020110031001469) （2020110031001469）
