中药与临床2025，Vol.16Issue(3)：8-15,8.
基于UPLC-Q-Orbitrap MS和分子网络技术快速鉴定远志与卵叶远志化学成分
Rapid identification of chemical constituents of Polygala tenuifolia Willd.and Polygala sibirica L.by UPLC-Q-Orbitrap MS and molecular networking
罗雅雯 1骆骄阳 2何春娇 1柯童炜 1秦旭华 3杨美华2
作者信息
- 1. 成都中医药大学 药学院,四川 成都 611137||中国医学科学院,北京协和医学院药用植物研究所中草药物质基础与资源利用教育部重点实验室,北京 100193
- 2. 中国医学科学院,北京协和医学院药用植物研究所中草药物质基础与资源利用教育部重点实验室,北京 100193
- 3. 成都中医药大学 药学院,四川 成都 611137
- 折叠
摘要
Abstract
Objective:To rapidly identify the chemical components in Polygala tenuifolia and Polygala sibirica,and explore the distribution of compounds in both plants.Methods:Ultra high performance liquid chromatography-Q-Electrostatic field orbital trap mass spectrometry(UPLC-Q-Orbitrap MS)was used to obtain the mass information of chemical constituents of P.tenuifolia and P.sibirica.The mass data was converted into mzXML format and uploaded to Global Natural Products Social(GNPS)platform for further analysis.The visual molecular networking result was obtained.Finally,compounds were rapidly identified by comparing them with controls and identifying excimer ion peaks and MS/MS ion fragments in the library.Results:In this study,34 compounds were rapidly identified,and the distribution of the potentially identified compounds in the two plants extracts was roughly the same.In addition,the fragmentation pathway of 3,6'-disinapoylsucrose analogues and flavonoid analogues was preliminarily speculated which provided a reference for further study of other types of compound iron fragmentation of P.tenuifolia and P.sibirica.Conclusion:The study elucidates the distribution of chemical components in P.tenuifolia and P.sibirica,providing a basis for the comprehensive development of Polygalae Radix resources.It also offers a method reference for the precise and rapid identification of the material basis in traditional Chinese medicines.关键词
UPLC-Q-Orbitrap MS/远志/卵叶远志/GNPS/分子网络Key words
UPLC-Q-Orbitrap MS/Polygala tenuifolia Willd/Polygala sibirica L./GNPS/molecular networking分类
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罗雅雯,骆骄阳,何春娇,柯童炜,秦旭华,杨美华..基于UPLC-Q-Orbitrap MS和分子网络技术快速鉴定远志与卵叶远志化学成分[J].中药与临床,2025,16(3):8-15,8.
