中药与临床2025，Vol.16Issue(3)：45-50,6.
基于Hedgehog信号通路的薏苡附子败酱散抗结直肠癌SW620细胞增殖和迁移的机制研究
Yiyi Fuzi Baijiang Powder suppresses proliferation and migration of colorectal cancer SW620 cells through regulation of Hedgehog signaling pathway
摘要
Abstract
Objective:This study aimed to explore the effects and mechanisms of Yiyi Fuzi Baijiang Powder(YFBP)on human colorectal cancer(CRC)SW620 cells.Methods:MTT assay,scratch assay and clonogenic assay were applied to detect the effects of different concentrations of YFBP(3.5,7.0 and 14.0 mg·mL-1)on the proliferation,migration and clonality of CRC SW620 cells.RT-qPCR and Western Blot were used to detect the potency of YFBP on Hedgehog signaling pathway activity.Results:YFBP inhibited the proliferation,migration and clonogenesis of SW620 cells.Constraining Hedgehog signaling cascade,YFBP decreased the mRNA and protein expression levels of SHH,SMO,Gli1,and c-Myc but increased the mRNA and protein levels of SuFu in CRC cells.Conclusion:Overall,YFBP might suppress colorectal cancer by down-regulating Hedgehog signaling pathway activity,inhibiting the proliferation and migration of SW620 cells.关键词
薏苡附子败酱散/Hedgehog信号通路/结直肠癌/SW620细胞Key words
Yiyi Fuzi Baijiang Powder/hedgehog signaling pathway/colorectal cancer/SW620 cells分类
医药卫生引用本文复制引用
王敏敏,彭宇辉,雷家荣,梁园菁,陶秋,明天琪,徐海波..基于Hedgehog信号通路的薏苡附子败酱散抗结直肠癌SW620细胞增殖和迁移的机制研究[J].中药与临床,2025,16(3):45-50,6.基金项目
国家自然科学基金面上项目(81573813) （81573813）
四川省自然科学基金面上项目(2023NSFSC0653) （2023NSFSC0653）
