中医药学报2025，Vol.53Issue(5)：18-22,5.DOI:10.19664/j.cnki.1002-2392.250090
黄芩素对人绒毛膜滋养层细胞增殖和血管生成的作用研究
Effect of Baicalein on Proliferation and Angiogenesis of Human Trophoblast Cells
摘要
Abstract
Objective:To investigate the effect of baicalein on angiogenesis in human trophoblast cells(HTR-8/Svneo).Methods:In this experiment,different doses of baicalein(0,5,10,20,40,and 100 nmol/L)were used to treat HTR-8/Svneo cells for 24 hours.Cell proliferation was assessed by the CCK-8 method to determine the low,medium,and high doses of baicalein.Subsequently,HTR-8/Svneo cells were treated with low,medium,and high doses of baicalein(20,40,and 100 nmol/L)for 24 hours.The supernatants of these cells were used to treat human umbilical vein endothelial cells(HUVECs)for 6 hours,and the tube formation ability of HUVECs was assessed by the matrigel tube formation assay.VEGF and PEDF levels in the supernatants of HTR-8/Svneo cells were measured using an ELISA kit.The mRNA expression levels of VEGF and PEDF in HTR-8/Svneo cells were measured by qRT-PCR.Results:Compared with the 0 nmol/L baicalein group,no significant change in cell proliferation was observed in the 5,10,and 20 nmol/L baicalein groups.However,cell proliferation activity significantly increased in the 40 nmol/L and 100 nmol/L baicalein groups(P<0.05).Compared with the 0 nmol/L baicalein group,the relative tube length and the number of cell junctions were significantly increased in the 40 nmol/L and 100 nmol/L baicalein groups(P<0.05).In comparison to the 0 nmol/L baicalein group,VEGF levels in the supernatant of HTR-8/Svneo cells were significantly increased in the 20,40,and 100 nmol/L baicalein groups(P<0.01),while PEDF levels significantly decreased(P<0.01).Furthermore,VEGF mRNA levels in HTR-8/Svneo cells were significantly increased in the 20,40,and 100 nmol/L baicalein groups(P<0.01),while PEDF mRNA levels were significantly decreased(P<0.01).Conclusion:Baicalein treatment can significantly promote angiogenesis in human trophoblast cells(HTR-8/Svneo).The underlying mechanism may involve the upregulation of VEGF levels and the inhibition of PEDF.关键词
黄芩素/绒毛膜滋养层细胞/血管生成/血管内皮生长因子/色素上皮衍生因子Key words
Baicalein/Trophoblast cells/Angiogenesis/Vascuar endothelial growth factor(VEGF)/Pigment epithelium-derived factor(PEDF)分类
医药卫生引用本文复制引用
陈小芳,帅纬纬,章琦,聂露,谢小花,黄淑晖..黄芩素对人绒毛膜滋养层细胞增殖和血管生成的作用研究[J].中医药学报,2025,53(5):18-22,5.基金项目
江西省中医药管理局科技计划项目(2021A391) （2021A391）
江西省卫生健康委科技计划(220210887) （220210887）
