中医药信息2025，Vol.42Issue(5)：1-8,14,9.DOI:10.19656/j.cnki.1002-2406.20250501
回阳固本汤对造血干细胞移植后肠道GVHD的防治作用及其网络药理学研究
The Preventive and Therapeutic Effect of Huiyang Guben Decoction on Intestinal Graft Versus Host Disease After Hematopoietic Stem Cell Transplantation and Its Network Pharmacology Research
摘要
Abstract
Objective:To explore the preventive and therapeutic effects of Huiyang Guben Decoction on intestinal graft versus host disease(GVHD)after hematopoietic stem cell transplantation.Methods:A retrospective analysis was conducted on 23 patients in a combined traditional Chinese and Western medicine treatment group(Group A)and 25 patients in a Western medicine-only treatment group(Group B)who underwent allogeneic hematopoietic stem cell transplantation,comparing the clinical manifestations of intestinal GVHD.Flow cytometry was used to compare cytokine expression levels before treatment and at various post-transplantation time points,both within and between groups.Network pharmacology was employed to predict the mechanisms by which Huiyang Guben Decoction intervenes in GVHD after transplantation.Results:Within 90 days post-transplant,the incidence of intestinal GVHD was significantly higher in Group B compared to Group A(P<0.05).No statistically significant difference was observed in IL-2 expression between groups or within Group A from pre-treatment to 120 days post-transplant(P>0.05),but Group B had higher IL-2 expression at 30 days compared to 90 days post-transplant(P<0.05).There was no significant difference in IL-4 expression between groups or within Group A from pre-treatment to 120 days(P>0.05),but Group B had higher IL-4 expression pre-treatment compared to 60 and 90 days post-transplant(P<0.05).Similarly,no significant difference in IL-17A expression was found between groups or within Group A from pre-treatment to 120 days(P>0.05),but Group B had higher IL-17A expression at pre-treatment compared to 30 days post-transplant(P<0.05),and at 30 days compared to 60 days(P<0.05).There were no significant differences in Th/Ts lymphocyte subsets between pre-treatment and 60 days post-transplant,but Group A had significantly higher values than Group B at 30 days post-transplant(P<0.05).Network pharmacology analysis identified 153 intersecting targets between Huiyang Guben Decoction and GVHD.Enrichment analysis revealed significant involvement in cancer pathways,chemical carcinogen-receptor activation,and IL-17 signaling.Molecular docking showed good binding activity of stigmasterol with matrix metalloproteinase-9(MMP-9).Conclusion:Huiyang Guben Decoction has certain therapeutic effects on intestinal GVHD after hematopoietic stem cell transplantation.From the cytokine perspective,it may enhance immune reconstitution and stability in GVHD patients post-transplantation.The mechanism may involve modulating cancer pathways,chemical carcinogen-receptor activation,and the IL-17 signaling pathway,particularly through the expression of MMP-9.关键词
回阳固本汤/造血干细胞移植/移植物抗宿主病/免疫重建/网络药理学Key words
Huiyang Guben Decoction/Hematopoietic stem cell transplantation/GVHD/Immune reconstitution/Network pharmacology引用本文复制引用
陈昌婷,余腾飞,朱千赜,王启,撒琪,冯帅,杨同华,胡芃..回阳固本汤对造血干细胞移植后肠道GVHD的防治作用及其网络药理学研究[J].中医药信息,2025,42(5):1-8,14,9.基金项目
国家自然科学基金项目(82004187) （82004187）
