中医药信息2025，Vol.42Issue(5)：15-20,6.DOI:10.19656/j.cnki.1002-2406.20250503
参芪益心方对大鼠心肌损伤内质网应激的影响
Effects of Shenqi Yixin Formula on Endoplasmic Reticulum Stress in Myocardial Injury of Rats
摘要
Abstract
Objective:To investigate the intervention effect of Shenqi Yixin Formula(SQYXF)on isoproterenol(ISO)-induced myocardial injury and endoplasmic reticulum stress.Methods:Forty SD rats were randomly divided into control group(Con group),model group(ISO group),trimetazidine treatment group(QM group),low-dose SQYXF treatment group(SL group),and high-dose SQYXF treatment group(SH group).The Con group received normal saline for 4 weeks,while all other groups received daily subcutaneous injections of ISO at 2 mg/(kg·d).From the third week onward,the QM group was administered trimetazidine suspension at 5 mg/(kg·d),the SL and SH groups received SQYXF decoction at 10 and 20 g/(kg·d)via gavage,respectively,and the Con and ISO groups received equivalent doses of normal saline.At the end of the 4-week experiment,rats were sacrificed,and myocardial tissues were collected to measure body weight,heart weight,and calculate the heart weight index.HE staining,Masson staining,and IHC staining were performed.Results:Compared with the Con group,the ISO group showed increased heart weight and heart weight index(P<0.05).Compared with the ISO group,the SH group exhibited increased heart weight but decreased heart weight index(P<0.05),while the QM and SL groups showed increased heart weight(P<0.05).HE staining revealed disordered myocardial structure and vacuolar degeneration of cardiomyocytes in the ISO group compared with the Con group.Compared with the ISO group,the QM group showed mild interstitial inflammatory cell infiltration and myocardial fiber edema,while the SL and SH groups exhibited reduced vacuolar degeneration and relatively regular myocardial fiber arrangement.Masson staining indicated enhanced myocardial fibrosis in the ISO group compared with the Con group,with significant improvement in the SH group.IHC staining demonstrated increased protein expression of inositol-requiring enzyme 1(IRE1),X-box binding protein 1(XBP1),cleaved caspase-3,and p53 upregulated modulator of apoptosis(PUMA)in the ISO group compared with the Con group,while the SH group showed significantly reduced expression of these proteins.Conclusion:SQYXF can reduce endoplasmic reticulum stress levels and improve myocardial injury,with the high-dose group showing the most significant effects.关键词
参芪益心方/心肌损伤/内质网应激Key words
Shenqi Yixin Formula/Myocardial injury/Endoplasmic reticulum stress引用本文复制引用
郭继芳,连一霏,赵莹,孔德谦,高凤敏,李作武..参芪益心方对大鼠心肌损伤内质网应激的影响[J].中医药信息,2025,42(5):15-20,6.基金项目
黑龙江省省属高等学校基本科研项目(2021-KYYWF-0512) （2021-KYYWF-0512）
黑龙江省卫生健康委科研项目(20210303010273) （20210303010273）
牡丹江市科技局科研项目(HT2022NS074) （HT2022NS074）
牡丹江医学院研究生导师科研专项计划项目(YJSPZX001) （YJSPZX001）
