组织工程与重建外科杂志2025，Vol.21Issue(2)：162-171,10.DOI:10.3969/j.issn.1673-0364.2025.02.010
先天性巨痣自发消退患者的单细胞测序分析
Single-cell sequencing of spontaneous regressed giant congenital melanocytic nevus(GCMN)
摘要
Abstract
Objective To explore the cellular turnover and biological mechanisms underlying the spontaneous regression of giant congenital melanocytic nevus(GCMN).Methods 10×single-cell RNA sequencing was performed on normal skin,depigmented nevus,and non-regressed nevus from patients with spontaneous regressed GCMN.Raw data were first used to quantify gene expression,followed by dimensionality reduction,clustering,cell population identification,and visualization.Cell populations were annotated based on differentially expressed genes(DEGs),and pathway enrichment and visualization of DEGs were carried out.Finally,histological staining was performed for validation.Results In GCMN,melanocytes constituted the largest proportion.During the regression process,the number of melanocytes decreased,while keratinocytes and fibroblasts increased.Immune cells exhibited a coexistence of both immune activation and suppression.In regressed tissues,melanocytes also expressed keratinocyte marker genes,while fibroblasts were enriched in pathways related to proliferation and extracellular matrix.Immunofluorescence staining revealed cells co-expressing SOX10 and Keratin,and pseudotime analysis indicated a transition of melanocytes into keratinocytes.Conclusion The regression of GCMN is a long-term chronic inflammatory process,during which melanocytes undergo transformation and detach from the epidermal layer.Fibroblasts contribute to tissue repair and reconstruction.关键词
先天性巨痣/单细胞测序/肿瘤微环境Key words
Giant congenital pigmented nevus/Single-cell sequencing/Tumor microenvironment分类
医药卫生引用本文复制引用
金佳敏,赖柏翰,顾婕妤,魏伯轩,谢峰..先天性巨痣自发消退患者的单细胞测序分析[J].组织工程与重建外科杂志,2025,21(2):162-171,10.基金项目
上海市科学技术委员会临床医学研究中心项目(22MC1940300). （22MC1940300）
