安徽中医药大学学报2025，Vol.44Issue(3)：50-56,7.DOI:10.3969/j.issn.2095-7246.2025.03.013
电针对糖尿病认知功能障碍大鼠海马区GLUTs、MCTs表达的影响
Effect of Electroacupuncture on the Expression of Glucose Transporters and Monocarboxylate Transporters in the Hippocampus of Rats with Diabetic Cognitive Impairment
摘要
Abstract
Objective To investigate the effect of electroacupuncture(EA)on the expression of glucose transporters(GLUTs)and monocarboxylate transporters(MCTs)and the structure of neurons in the hippocampus of rats with diabetic cognitive im-pairment(DCI),and to discuss the possible mechanism of EA in improving DCI.Methods Sprague-Dawley rats were random-ly divided into blank group with 10 rats and high-fat diet group with 90 rats.The rats in the high-fat diet group were used to establish a model of diabetes,and at 9 weeks after successful modeling,the Morris water maze test was used to identify 20 rats with DCI,which were randomly divided into EA group and model group,with 10 rats in each group.The rats in the EA group were given acupuncture at Yishu,Zusanli,and Neiting points and EA at Zusanli and Neiting points for 15 minutes each time,once a day for 6 consecutive days,followed by 1 day of rest,with a course of treatment of 4 weeks.The rats in the other two groups were fixed without any other treatment.Fasting blood glucose was measured for the three groups of mice before model-ing,after modeling,and after EA intervention;the Morris water maze test was used to assess cognitive function;HE staining was used to observe the pathomorphological changes;Western blot was used to measure the protein expression levels of GLUT1,GLUT3,MCT1,MCT2,and MCT4 in the hippocampus,and immune of luorescence assay was used to measure the fluorescence intensities of GLUT1,GLUT3,MCT1,MCT2,and MCT4 in the hippocampus.Results EA significantly re-duced fasting blood glucose(P＜0.05)and escape latency(P＜0.05),increased the number of platform crossings(P＜0.05),improved the morphology of neuronal cells in the hippocampus,and increased the protein expression levels and fluorescence in-tensities of GLUT1,GLUT3,MCT1,MCT2,and MCT4(P＜0.05)in the hippocampus.Conclusion EA can improve the learning and memory abilities of DCI rats,possibly by upregulating the expression of GLUTs and MCTs and enhancing the utili-zation of glucose and monocarboxylate in the hippocampus.关键词
糖尿病认知障碍/葡萄糖转运体/单羧酸转运体/神经元/电针Key words
Diabetic cognitive impairment/Glucose transporters/Monocarboxylate transporters/Electroacupuncture分类
医药卫生引用本文复制引用
宋蝴蝶,袁爱红,叶敏,张乐乐,李飞跃,李群威,韦艳,解鸿宇..电针对糖尿病认知功能障碍大鼠海马区GLUTs、MCTs表达的影响[J].安徽中医药大学学报,2025,44(3):50-56,7.基金项目
安徽省2023年度中央财政中医药事业传承与发展专项资金项目(皖中医药发展秘[2023]23号) （皖中医药发展秘[2023]23号）
第九批安徽省"特支计划"(皖组办字[2023]35号) （皖组办字[2023]35号）
第五批全国中医临床优秀人才研修项目(国中医药人教函[2022]1号) （国中医药人教函[2022]1号）
