电针对糖尿病认知功能障碍大鼠海马区GLUTs、MCTs表达的影响OA
Effect of Electroacupuncture on the Expression of Glucose Transporters and Monocarboxylate Transporters in the Hippocampus of Rats with Diabetic Cognitive Impairment
目的 观察电针对糖尿病认知功能障碍(diabetes cognitive impairment,DCI)大鼠海马区葡萄糖转运蛋白(glucose transporters,GLUTs)、单羧酸转运蛋白(monocarboxylate transporters,MCTs)表达及神经元结构的影响,探讨电针改善DCI的可能机制.方法 将SD大鼠随机分为空白组10只,高脂饲料组90只.高脂饲料组大鼠用于构建糖尿病模型,模型复制成功9周后,再采用Morris水迷宫实验筛选出DCI大鼠20只,随机分为电针组和模型组,每组10只.电针组大鼠施以"胰俞""足三里"和"内庭"穴针刺治疗,其中"足三里""内庭"穴给予电针治疗,每日1次,每次15 min,连续治疗6 d后休息1d,持续4周.其余两组大鼠只做固定不进行治疗.分别于模型复制前、模型复制后及电针干预后检测3组大鼠空腹血糖值,Morris水迷宫实验评定大鼠认知功能,苏木精—伊红染色法观察大鼠海马组织病理形态改变,Western blot法检测大鼠海马组织GLUT1、GLUT3、MCT1、MCT2、MCT4表达水平,免疫荧光法观察大鼠海马组织GLUT1、GLUT3、MCT1、MCT2、MCT4平均荧光强度.结果 电针可以显著降低DCI大鼠空腹血糖值(P＜0.05),减少逃避潜伏期(P＜0.05),增加穿越平台次数(P＜0.05),改善海马区神经元细胞形态,提高海马组织中GLUT1、GLUT3、MCT1、MCT2、MCT4表达水平及平均荧光强度(P＜0.05).结论 电针可以增强DCI大鼠学习记忆能力,其作用可能与上调GLUTs、MCTs表达,提高海马区葡萄糖与单羧酸盐的利用有关.
Objective To investigate the effect of electroacupuncture(EA)on the expression of glucose transporters(GLUTs)and monocarboxylate transporters(MCTs)and the structure of neurons in the hippocampus of rats with diabetic cognitive im-pairment(DCI),and to discuss the possible mechanism of EA in improving DCI.Methods Sprague-Dawley rats were random-ly divided into blank group with 10 rats and high-fat diet group with 90 rats.The rats in the high-fat diet group were used to establish a model of diabetes,and at 9 weeks after successful modeling,the Morris water maze test was used to identify 20 rats with DCI,which were randomly divided into EA group and model group,with 10 rats in each group.The rats in the EA group were given acupuncture at Yishu,Zusanli,and Neiting points and EA at Zusanli and Neiting points for 15 minutes each time,once a day for 6 consecutive days,followed by 1 day of rest,with a course of treatment of 4 weeks.The rats in the other two groups were fixed without any other treatment.Fasting blood glucose was measured for the three groups of mice before model-ing,after modeling,and after EA intervention;the Morris water maze test was used to assess cognitive function;HE staining was used to observe the pathomorphological changes;Western blot was used to measure the protein expression levels of GLUT1,GLUT3,MCT1,MCT2,and MCT4 in the hippocampus,and immune of luorescence assay was used to measure the fluorescence intensities of GLUT1,GLUT3,MCT1,MCT2,and MCT4 in the hippocampus.Results EA significantly re-duced fasting blood glucose(P＜0.05)and escape latency(P＜0.05),increased the number of platform crossings(P＜0.05),improved the morphology of neuronal cells in the hippocampus,and increased the protein expression levels and fluorescence in-tensities of GLUT1,GLUT3,MCT1,MCT2,and MCT4(P＜0.05)in the hippocampus.Conclusion EA can improve the learning and memory abilities of DCI rats,possibly by upregulating the expression of GLUTs and MCTs and enhancing the utili-zation of glucose and monocarboxylate in the hippocampus.
宋蝴蝶;袁爱红;叶敏;张乐乐;李飞跃;李群威;韦艳;解鸿宇
安徽中医药大学第一临床医学院,安徽 合肥 230031安徽中医药大学第一附属医院,安徽 合肥 230031安徽中医药大学第一临床医学院,安徽 合肥 230031安徽中医药大学第一临床医学院,安徽 合肥 230031安徽中医药大学第一临床医学院,安徽 合肥 230031安徽中医药大学第一临床医学院,安徽 合肥 230031安徽中医药大学第一临床医学院,安徽 合肥 230031安徽中医药大学第一附属医院,安徽 合肥 230031
临床医学
糖尿病认知障碍葡萄糖转运体单羧酸转运体神经元电针
Diabetic cognitive impairmentGlucose transportersMonocarboxylate transportersElectroacupuncture
《安徽中医药大学学报》 2025 (3)
50-56,7
安徽省2023年度中央财政中医药事业传承与发展专项资金项目(皖中医药发展秘[2023]23号)第九批安徽省"特支计划"(皖组办字[2023]35号)第五批全国中医临床优秀人才研修项目(国中医药人教函[2022]1号)
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