安徽中医药大学学报2025，Vol.44Issue(3)：57-64,8.DOI:10.3969/j.issn.2095-7246.2025.03.014
从分子网络到代谢调控探究川芎和赤芍治疗缺血性脑卒中的作用机制
Mechanism of Action of Rhizoma chuanxiong and Radix Paeoniae rubra in Treatment of Ische-mic Stroke:A Study Based on Molecular Network and Metabolic Regulation
摘要
Abstract
Objective To investigate the therapeutic effect and potential mechanism of Rhizoma chuanxiong and Radix Paeoniae rubra used alone or in combination in the treatment of ischemic stroke(IS),and to compare the clinical effect of these two drugs.Methods A rat model of middle cerebral artery occlusion/reperfusion(MCAO/R)was established to simulate vascular occlusion in human IS and the process of thrombolysis and reperfusion.Network pharmacology was used to predict the potential action targets of the two drugs and related pathways,and the method of UHPLC-Q-TOF-MS/MS-based non-targeted metabo-lomics was used to investigate changes in the metabolic profile of brain tissue in rats with cerebral infarction.Results Both Rhizoma chuanxiong and Radix Paeoniae rubra improved pathological changes in MCAO/R rats,and Radix Paeoniae rubra showed a more significant effect.Network pharmacology and metabolomics analysis showed that the two drugs exerted an effect by influencing various pathways including lipid metabolism,inflammatory response,cell apoptosis,and energy metabolism.Conclusion Both Rhizoma chuanxiong and Radix Paeoniae rubra can effectively improve brain injury in IS rats,and Radix Pae-oniae rubra shows a more significant effect.The two drugs exert a neuroprotective effect through multiple targets and path-ways,and there is a synergistic effect between these two drugs,which provides new ideas and potential targets for the tradi-tional Chinese medicine treatment of IS.关键词
川芎/赤芍/药对/缺血性脑卒中/网络药理学/代谢组学Key words
Rhizoma chuanxiong/Radix Paeoniae rubra/Drug combination/Ischemic stroke/Network pharmacology/Metabolomics分类
中医学引用本文复制引用
甘芮溪,王琮,汪俊松,陈卫东..从分子网络到代谢调控探究川芎和赤芍治疗缺血性脑卒中的作用机制[J].安徽中医药大学学报,2025,44(3):57-64,8.基金项目
安徽省高校科研计划项目团队项目(自然科学)(2022AH010036) （自然科学）
国家中医药管理局高水平重点学科中药资源学(药用植物学)项目(zyyzdxk-2023095) （药用植物学）
省部共建安徽道地中药材品质提升协同创新中心(教科信厅函[2022]4号) （教科信厅函[2022]4号）
