安徽中医药大学学报2025，Vol.44Issue(3)：75-81,7.DOI:10.3969/j.issn.2095-7246.2025.03.016
芪黄煎剂对脂多糖诱导的巨噬细胞M1型极化及p38MAPK/NF-κB通路的影响
Effect of Qihuang Decoction on Lipopolysaccharide-induced M1 Macrophage Polarization and the p38 Mitogen-activated Protein Kinase/Nuclear Factor-Kappa B Pathway
摘要
Abstract
Objective To investigate the effect of Qihuang Decoction on lipopolysaccharide(LPS)-induced M1 macrophage po-larization and the p38 mitogen-activated protein kinase(p38MAPK)/nuclear factor-kappa B(NF-κB)signaling pathway.Methods Sprague-Dawley rats were given Qihuang Decoction by gavage to prepare drug-containing serum.RAW264.7 macro-phages were divided into control group,LPS group,LPS+Qihuang Decoction group,Qihuang Decoction+LPS group,LPS+NF-κB inhibitor group,LPS+p38MAPK inhibitor group,LPS+NF-κB inhibitor+Qihuang Decoction group,and LPS+p38MAPK inhibitor+Qihuang Decoction group.All groups except the control group were given LPS to induce RAW264.7 macrophages for 24 hours.Flow cytometry was used to detect the polarization of macrophages;ELISA was used to measure the levels of interleukin-6(IL-6),interleukin-1β(IL-1β),and tumor necrosis factor-α(TNF-α)in supernatant;RT-qPCR was used to measure the mRNA expression levels of IL-6,IL-1β,TNF-α,NF-κB p65,p38MAPK,inhibitor of nuclear factor-kappa B alpha(IκBα),Toll-like receptor 4(TLR4),and TNF receptor-associated factor 6(TRAF6),and Western blot was used to measure the protein expression levels of NF-κB p65,p38MAPK,IκBα,TLR4,and TRAF6.Results Compared with the con-trol group,the LPS group had a significant increase in the expression level of CD86 and a significant reduction in the expression level of CD206 on cell surface(P＜0.05);compared with the LPS group,the LPS+Qihuang Decoction group had a significant reduction in the expression level of CD86 and a significant increase in the expression level of CD206(P＜0.05);compared with the LPS+NF-κB inhibitor group,the LPS+NF-κB inhibitor+Qihuang Decoction group had significant reductions in the ex-pression levels of CD86 and CD206(P＜0.05).Qihuang Decoction and NF-κB inhibitor significantly reduced the levels of IL-6,IL-1β,and TNF-α in the supernatant of RAW264.7 macrophages induced by LPS(P＜0.05),and Qihuang Decoction enhanced the effect of NF-κB inhibitor(P＜0.05).Qihuang Decoction and NF-κB inhibitor significantly reduced the mRNA and protein expression levels of IL-6,IL-1β,TNF-α,NF-κB p65,p38MAPK,and IκBα in RAW264.7 macrophages induced by LPS(P＜0.05),and Qihuang Decoction enhanced the effect of NF-κB inhibitor(P＜0.05);NF-κB inhibitor could not significantly re-duce the mRNA and protein expression levels of TLR4 and TRAF6 in RAW264.7 macrophages induced by LPS(P＞0.05),while Qihuang Decoction significantly enhanced the effect of NF-κB inhibitor(P＜0.05).Conclusion The drug serum contai-ning Qihuang Decoction can inhibit inflammatory response in macrophages induced by LPS,possibly by regulating the p38MAPK/NF-κB signaling pathway to inhibit M1 macrophage polarization.关键词
胃切除术/巨噬细胞极化/p38MAPK/NF-κB信号通路/芪黄煎剂Key words
Gastrectomy/Macrophage polarization/p38 Mitogen-activated protein kinase/nuclear factor-kappa B signaling pathway/Qihuang Decoction分类
中医学引用本文复制引用
刘海韦,张琦,于庆生,刘举达,舒亚倩,刘旭,齐伟,黄龙,彭辉..芪黄煎剂对脂多糖诱导的巨噬细胞M1型极化及p38MAPK/NF-κB通路的影响[J].安徽中医药大学学报,2025,44(3):75-81,7.基金项目
安徽高校自然科学研究重点项目(2023AH050751) （2023AH050751）
安徽省卫生健康科研项目(AHWJ2023A20362) （AHWJ2023A20362）
安徽省临床医学研究转化专项(202427b10020025) （202427b10020025）
