草业科学2025，Vol.42Issue(4)：823-832,10.DOI:10.11829/j.issn.1001-0629.2023-0536
响应面法优化沙打旺总生物碱提取工艺及活性研究
Optimization of extraction process and activity of total alkaloids from Astragalus adsurgens using response surface methodology
摘要
Abstract
Astragalus adsurgens is an excellent forage containing alkaloids.This study employed Box-Behnken response surface methodology to optimize the extraction process of total alkaloids from A.adsurgens by investigating three main factors:ethanol concentration,liquid-to-solid ratio,and extraction time.Antioxidant and antibacterial activity were also determined.Results indicated that the optimal extraction conditions for total alkaloids were an ethanol concentration of 80.93％,a liquid-to-material ratio of 1∶21.36,and a 2.75 h extraction time.The predicted maximum yield is 0.14％.Total alkaloids in A.adsurgens had strong antioxidant capacity.As the concentration of A.adsurgens total alkaloids increased,the clearance rate gradually increased,and the total antioxidant capacity gradually strengthened before decreasing.The minimum inhibitory concentrations for Staphylococcus aureus and Escherichia coli were 100 and 50 mg·mL-1,respectively.The results of this study have important significance for the development and utilization of total alkaloids of A.adsurgens and the promotion of resistant culture.关键词
直立黄芪/提取物/乙醇浓度/响应面法/抗氧化活性/自由基清除能力/抑菌Key words
Astragalus adsurgens/extractive/ethanol concentration/response surface method/antioxidant activity/free radical scavenging ability/antibacterial引用本文复制引用
胡亚娜,李洋,田雨,孙泽威..响应面法优化沙打旺总生物碱提取工艺及活性研究[J].草业科学,2025,42(4):823-832,10.基金项目
吉林省重大科技专项(20230303008SF) （20230303008SF）
