草原与草坪2025，Vol.45Issue(2)：24-31,8.DOI:10.13817/j.cnki.cyycp.2025.02.003
低温胁迫对WL系列紫花苜蓿品种抗氧化生理的影响
Antioxidant characteristics and cold resistance evaluation of W L series alfalfa varieties under low temperature stress
摘要
Abstract
[Objective]To investigate the physiological responses of alfalfa to low-temperature stress and to identifyvarieties with high cold resistance.[Method]Twelve alfalfa varieties of the 'WL' series from the United States were selected as test materials and planted in the Horqin sandy land.Alfalfa overwintering materials were dug prior to overwintering in order to simulate 4℃(CK)and-20℃low temperature stress treatment.Malondialde-hyde(MDA)content and the activities of superoxide dismutase(SOD),catalase(CAT),and peroxidase(POD)in the root neck were measured.Cold resistance was evaluated using correlation analysis,principal component analysis,and membership function analysis.[Result]The results showed that compared with the control,the activities of SOD,CAT and POD and the content of MDA in all 12 alfalfa varieties increased under low temperature stress.Com-pared with other varieties of alfalfa,WL168HQ,WL298HQ and WL354HQ showed relatively higher antioxidant en-zyme activity and lower MDA content.Correlation analysis of antioxidant enzyme activities and malondialdehyde con-tent revealed that CAT and SOD had significant negative correlations with MDA under low-temperature stress(P<0.05).[Conclusion]Based on membership function analysis,the cold resistance of the 12 varieties was:WL168HQ>WL329HQ>WL298HQ>WL319HQ>WL349HQ>WL358HQ>WL366HQ>WL377HQ>WL363HQ>WL354HQ>WL343HQ>WL440HQ.It is recommended to choose WL168HQ,WL298HQ,WL319HQ,and WL329HQ alfalfa varieties for planting WL series alfalfa varieties in Horqin sandy land,due to their low fall dormancy level and high cold resistance.关键词
紫花苜蓿/品种/低温胁迫/抗氧化特性/抗寒性/MDAKey words
alfalfa/varieties/low temperature stress/antioxidant properties/cold resistance/MDA分类
农业科技引用本文复制引用
刘泽宇,隋可,袁梓桐,张玉霞,刘庭玉,单新河,张智勇,王显国..低温胁迫对WL系列紫花苜蓿品种抗氧化生理的影响[J].草原与草坪,2025,45(2):24-31,8.基金项目
国家牧草产业技术体系项目(CARS-34),内蒙古自治区直属高校专项(GXKY22018),内蒙古自治区生态农业国家民委重点实验室开放基金项目(MDK2023095) （CARS-34）
