草原与草坪2025，Vol.45Issue(2)：52-61,10.DOI:10.13817/j.cnki.cyycp.2025.02.006
盐胁迫下草地早熟禾出苗期可培养细菌多样性与促生功能研究
Diversity and growth-promoting function of culturable bacteria at seedling stage of Poa pratensis under salt stress
摘要
Abstract
[Objective]To analyze the diversity of bacterial community cultured by Poa pratensis under 200 mmol/L NaCl stress,and screen strains with both salt tolerance and growth-promoting functions,so as to provide theoretical basis and strain resources for biological improvement of saline soil.[Method]The culturable bacteria from stem,leaf and root of Youmei and Xinyue cultivars were isolated by dilution coating method,and the strains respon-sive to salt stress were screened by combining their salt tolerance,nitrogen fixation,phosphorus dissolution,potas-sium dissolution,IAA production and extracellular enzyme production abilities.[Result]A total of 27 culturable bac-teria belong to three phyla and 13 genera.In the control group,the dominant genera in the above-ground parts of Youmei and Xinyue were Pseudomonas and Chryseobacterium,respectively,and the dominant genera in the under-ground parts were Pseudomonas and Agrobacterium,respectively.Compared with the control group,the number of Pseudomonas increased by 61.78%and 51.67%to 7.64×103 CFU/g and 4.8×103 CFU/g in the Youmei and Xinyue salt stress groups,respectively.Among the 27 bacteria strains,L7(Pseudomonas),L27 and B2(Bacillus)have salt tolerance thresholds as high as 20%,and have various growth-promoting functions.[Conclusion]Salt stress has a significant effect on the community structure of culturable bacteria in grassland Poa pratensis tissues.Strains L7,L27 and B2 were screened out as salt-tolerant strains with excellent growth-promoting functions,which laid a theoretical foundation and bacterial resources for improving the bioremediation function of Poa pratensis in salinized grassland.关键词
草地早熟禾/盐胁迫/植物细菌/多样性/耐盐性/促生功能Key words
Poa pratensis/salt stress/plant bacteria/diversity/salt tolerance/promotive function分类
农业科技引用本文复制引用
丁津,朱环,王晓芬..盐胁迫下草地早熟禾出苗期可培养细菌多样性与促生功能研究[J].草原与草坪,2025,45(2):52-61,10.基金项目
甘肃省林业和草原局2024年度自列科技项目(2024kj86) （2024kj86）
甘肃省教育厅青年博士支持项目(2025QB-051) （2025QB-051）
