草原与草坪2025，Vol.45Issue(2)：70-82,13.DOI:10.13817/j.cnki.cyycp.2025.02.008
外源H2O2对盐碱胁迫下万寿菊光合能力和抗性生理的影响
Effects of exogenous H2O2 on photosynthetic capacity and resistance physiology of marigold under saline-alkali stress
摘要
Abstract
[Objective]To investigate the effects of exogenous hydrogen peroxide(H2O2)on the photosynthetic characteristics and physiological functions of potted marigolds(Tagetes erecta)under mixed saline-alkaline stress,and to identify the optimal exogenous H2O2 concentration for enhancing stress resistance.[Method]Five concentra-tions of exogenous H2O2 were applied under control(CK)and mixed saline-alkaline stress conditions:T1(0 μmol/L),T2(50 μmol/L),T3(100 μmol/L),T4(200 μmol/L)and T5(300 μmol/L).Phenotypic observations were recorded,and physiological parameters including chlorophyll content,leaf osmotic substances,relative electrical conductivity(REC),malondialdehyde(MDA),chlorophyll fluorescence parameters,antioxidant substance content and root conformation parameters were measured.Correlation and principal component analyses were conducted for comprehensive analysis.[Result]The results showed that the REC,MDA,leaf osmotic substances and root confor-mation parameters of marigold leaves exhibited an increasing trend with the prolongation of saline and alkaline stress,while the chlorophyll content and leaf fluorescence parameters showed a decreasing trend.The magnitude of the changes of each index was reduced after exogenous H2O2 treatment,with clear concentration-dependent responses.Among all treatments,T4(200 μmol/L)resulted in smallest decrease in chlorophyll content,leaf fluorescence param-eters,and root conformation parameters,as well as the lowest increase in MDA and REC.T4 also showed the great-est enhancement in leaf osmotic regulation.After comprehensive evaluation,the rankings of the treatments were CK>T4>T3>T2>T5>T1.[Conclusion]It was shown that exogenous H2O2 could promote the growth of the root system of marigold,enhance the photosynthetic and osmotic regulating ability of the leaves,and activate the activity of antioxidant enzymes,which could slow down the damage of membrane lipid peroxidation.Ultimately alleviate the damage of saline and alkaline stress on marigold with the most effective concentration being 200 μmol/L.关键词
外源H2O2/万寿菊/盐碱胁迫/生理特性/光合特性Key words
exogenous H2O2/marigold/saline-alkali stress/physiological characteristics/photosynthetic charac-teristics分类
农业科技引用本文复制引用
朱进威,蒋倩,张旺君,刘兴,王燕,缐旭林..外源H2O2对盐碱胁迫下万寿菊光合能力和抗性生理的影响[J].草原与草坪,2025,45(2):70-82,13.基金项目
甘肃省高等学校创新基金项目(022B-098) （022B-098）
