分析化学2025，Vol.53Issue(5)：681-688,8.DOI:10.19756/j.issn.0253-3820.251081
具备群体感应信号分泌和接收能力的人造细胞的构建及其信号转导研究
Construction of Artificial Cells with the Ability to Secrete and Receive Quorum Sensing Signals and Study on Their Signal Transduction
摘要
Abstract
The bottom-up construction of artificial cells to mimic signal transduction is of great significance for a deep understanding of the communication networks of complex living systems.The dynamic coupling of signal secretion and response among artificial cells poses an important challenge.In this work,two types of artificial cells were constructed through a bio-artificial hybrid strategy.One was an artificial cell as an AI-2 signal sender(sender),which contained bacteria capable of autonomously synthesizing AI-2 signaling molecules.The other was an artificial cell as an AI-2 signal receiver(receiver),which contained bacteria expressing green fluorescent protein in response to the corresponding AI-2 signal.When the ratio of senders to receivers was 4∶1,the fluorescent signal of the receivers gradually increased over time and reached a plateau at 240 minutes.The artificial cells capable of sending and receiving quorum sensing signaling molecules constructed in this work laid a foundation for the study of complex signal communication within artificial cell populations.关键词
大肠杆菌/群体感应/信号分子/人造细胞Key words
Escherichia coli/Quorum sensing/Signal molecules/Artificial cells引用本文复制引用
杜行,赵晶晶,李书彬,韩晓军..具备群体感应信号分泌和接收能力的人造细胞的构建及其信号转导研究[J].分析化学,2025,53(5):681-688,8.基金项目
国家自然科学基金项目(Nos.22374033,22174031)和黑龙江省自然科学基金项目(No.ZD2022B001)资助. Supported by the National Natural Science Foundation of China(Nos.22374033,22174031)and the Natural Science Foundation of Heilongjiang Province,China(No.ZD2022B001). （Nos.22374033,22174031）
