分析化学2025，Vol.53Issue(5)：749-757,9.DOI:10.19756/j.issn.0253-3820.241387
原生态样品原位萃取离子化技术及小型装置研究
Research on In-Situ Extractive Ionization for Original Ecological Samples and Its Miniature Device
摘要
Abstract
Current ambient mass spectrometry ionization often requires external auxiliary equipment such as high-voltage power supply,gas cylinder,and syringe pump.Moreover,the process of sample preparation is cumbersome,and the experimental operations are complex,which makes it difficult to adapt to real-time on-site detection.In this work,a novel method was proposed,in which direct sampling of raw samples,online extraction of interest analytes,and ionization of target molecules were integrated into a single unit.With the developed method,the in-situ extraction and nano-electrospray ionization for both liquid and solid raw samples were achieved.Also,a handheld ion source and its pose adjustment device were developed,and the position and angle parameters were subsequently optimized.The performance of the ionization device was tested using standard solutions of caffeine and reserpine.The limits of detection(LODs)were 0.08 μg/L and 0.14 μg/L,with relative standard deviations(RSDs)≤3.7% and≤5.6%,respectively,indicating that the device possessed high sensitivity and stability.Using this device,three different concentrations of reserpine standard solutions were continuously tested for five days.The intra-day RSDs were consistently≤4.7% and the inter-day RSDs were all≤10.3%,showing the good working stability of the device.Without any pretreatment,a rapid qualitative detection of medicinal components including astragaloside II and cycloastragenol in five traditional Chinese medicines was carried out,with RSDs≤8.0% and≤7.1%,respectively.Additionally,rapid qualitative detection of gallic acid,a medicinal component,in white peony roots,and hypaphorine as well as quercetin in cowherb seeds were carried out,with RSDs≤7.0%,≤6.4% and≤6.1%,respectively.These results demonstrated that the ionization technology and device exhibited good stability during qualitative detection of raw samples.关键词
直接质谱/原位萃取离子化/小型装置/中药检测Key words
Ambient mass spectrometry/In-situ extractive ionization/Miniature device/Traditional Chinese medicine detection引用本文复制引用
董晓峰,刘锋,薛又菡,叶喜德,王双龙..原生态样品原位萃取离子化技术及小型装置研究[J].分析化学,2025,53(5):749-757,9.基金项目
江西省自然科学基金项目(Nos.20242BAB25139,20242BAB25131)、江西省重点研发计划项目(No.20232BBG70013)和江西省自然科学基金青年基金项目(No.20224BAB216007)资助. Supported by the Project of Jiangxi Provincial Natural Science Foundation(Nos.20242BAB25139,20242BAB25131),the Project of Jiangxi Provincial Key Research and Development Plan(No.20232BBG70013)and the Youth Fund Project of Jiangxi Provincial Natural Science Foundation(No.20224BAB216007). （Nos.20242BAB25139,20242BAB25131）
