分析化学2025，Vol.53Issue(5)：785-793,9.DOI:10.19756/j.issn.0253-3820.241368
基于滚环扩增及盐酸小檗碱快速检测p53基因
Rapid Detection of p53 Gene Based on Rolling Circle Amplification and Berberine Hydrochloride
摘要
Abstract
In this work,a rapid and label-free sensing platform was designed for visual detection of p53 gene.The rolling circle amplification(RCA)process of the assay platform was activated by p53 gene to produce long DNA-wires,which could bound with berberine hydrochloride(BBH)and further enhanced its fluorescence.This method showed high sensitivity with a low detection limit of 5.63 pmol/L,and high specificity toward p53 gene over other interference materials,even for single-base mutation gene.The method could realize the visual detection of targets under the illumination of a UV lamp.In addition,the designed fluorescence detection platform was successfully applied to p53 gene analysis in 10% fetal bovine serum samples,and the relative standard deviation and the recoveries were 0.1% -1.2% and 99.5% -104.7%,respectively.This approach had satisfactory characteristics,such as low cost,label-free,rapidity,high sensitivity,good selectivity and anti-interference ability,and reliable detection capability for complex practical samples,demonstrating a promising prospect in the diagnosis and treatment of diseases,especially for cancer.关键词
p53基因/滚环扩增/免标记/盐酸小檗碱/荧光检测Key words
p53 gene/Rolling circle amplification/Label-free/Berberine hydrochloride/Fluorescence detection引用本文复制引用
张京燕,张钰苹,谢林惠,周鸿,罗思瑶,罗荧萍..基于滚环扩增及盐酸小檗碱快速检测p53基因[J].分析化学,2025,53(5):785-793,9.基金项目
国家自然科学基金项目(No.82104116)、南充市"市校合作"专项科研项目(No.20SXQT0326)和四川省大学生创新创业项目(No.S202210634069)资助. Supported by the National Natural Science Foundation of China(No.82104116),the ″Government-Universities″ Specific Cooperative Scientific Research Project of Nanchong(No.20SXQT0326)and the Innovation and Entrepreneurship Training Program for College Students(No.S202210634069). （No.82104116）
