分析化学2025，Vol.53Issue(5)：814-822,9.DOI:10.19756/j.issn.0253-3820.231377
纳米超表面等离子体共振芯片耦合金纳米颗粒的免疫竞争法检测恩诺沙星
Competitive Immunoassay for Detection of Enrofloxacin Based on Metasurface Plasma Resonance Chip Coupled with Gold Nanoparticles
摘要
Abstract
Risks of food safety induced by small molecule drug residues in animal food and environment have become an increasing public concern,so it is necessary to develop highly sensitive and easy-to-operate techniques to detect small molecules.Herein,a metasurface plasma resonance(MetaSPR)sensor chip coupled with gold nanoparticles(AuNPs)was developed for detection of enrofloxacin(ENR)based on competitive immunoassay.The detection range of the sensor for ENR was 0.025-3.2 ng/mL,and the detection limit(3σ)was 20 pg/mL.The biosensor showed excellent performance including high selectivity,good stability,ease to operate and high throughput,etc.The developed method was applied to detection of ENR residues in real samples,with recoveies of 96.0% -105.0%.The proposed sensing strategy provided new technique reference for detection of other small molecules in the field of residue analysis in food safety and environment monitoring.关键词
纳米超表面等离子体共振/生物芯片/金纳米颗粒/免疫竞争/恩诺沙星Key words
Meta-surface plasma resonance/Biochip/Gold nanoparticles/Competitive immunoassay/Enrofloxacin引用本文复制引用
冀威昊,樊洪利,龚蕾,黄丽萍,范小龙,胡家勇,周陶鸿,刘钢..纳米超表面等离子体共振芯片耦合金纳米颗粒的免疫竞争法检测恩诺沙星[J].分析化学,2025,53(5):814-822,9.基金项目
国家市场监督管理总局科技计划项目(No.2023MK087)和国家重点研发计划项目(No.2020YFC0861900)资助. Supported by the Scientific Technology Project of State Administration for Market Regulation(No.2023MK087)and the National Key Research and Development Project of China(No.2020YFC0861900). （No.2023MK087）
