分子影像学杂志2025，Vol.48Issue(5)：529-534,6.DOI:10.12122/j.issn.1674-4500.2025.05.01
癌细胞膜修饰的新型多功能纳米探针在乳腺癌中的应用
Application of a novel multifunctional nanoprobes modified by cancer cell membrane in breast cancer
摘要
Abstract
Objective To develop a novel multifunctional integrated diagnostic and therapeutic nanoprobes targeting breast cancer,MnO2@DOX@Cancer Cell Membrane(MDC),evaluate its basic properties,and explore its magnetic resonance imaging(MRI)capabilities and chemotherapy effects.Methods MnO2 with a favorable morphology was synthesized.The cell membrane of 4T1 mouse breast cancer cells was obtained using chemical lysis and ultrasonic fragmentation techniques.A lipid extrusion method was used to synthesize the cell membrane-modified nanoprobes MDC loaded with doxorubicin(DOX).Key properties of MDC,including morphology,surface potential,and particle size,were characterized,and its MRI capability was explored.The cytotoxic effects of MDC at different concentrations on tumor cells were observed to assess its inhibitory effect on breast cancer tumors in chemotherapy.The integrated diagnostic and therapeutic functions at the cellular level were also verified.Results The nanoprobes MDC with a nanoflower-like structure were successfully synthesized,with a Zeta potential of-20±1.5 mV and an average particle size of 151.2 nm.Transmission electron microscopy(TEM)imaging confirmed successful coating of the cell membrane onto the nanoprobes.MRI results showed that MDC exhibited excellent MRI T1 imaging ability in a simulated tumor microenvironment.Laser confocal microscopy experiments further confirmed that MDC had excellent homologous targeting capability.Cytotoxicity experiments indicated that MDC significantly killed cancer cells.Conclusion This study successfully synthesized a multifunctional integrated diagnostic and therapeutic nanoprobes MDC,which exhibited excellent homologous targeting and anti-tumor effects in chemotherapy,significantly enhanced MRI imaging,and demonstrated integrated diagnostic and therapeutic functions at the cellular level.关键词
癌细胞膜/纳米探针/乳腺癌/化学治疗/磁共振成像Key words
cancer cell membrane/nanoprobe/breast cancer/chemotherapy therapy/magnetic resonance imaging引用本文复制引用
覃珩依,杨彩霞,李祥林,高瑜婧,崔婷,丁琦梅,王佳伟,纪抒含,陶晋,魏俊辉,王婷婷..癌细胞膜修饰的新型多功能纳米探针在乳腺癌中的应用[J].分子影像学杂志,2025,48(5):529-534,6.基金项目
国家自然科学基金(62176181) （62176181）
山东省重点研发计划(2018YFJH0501) （2018YFJH0501）
山东省大学生创新创业训练计划(202310440237)Supported by National Natural Science Foundation of China(62176181) （202310440237）
