分子影像学杂志2025，Vol.48Issue(5)：562-568,7.DOI:10.12122/j.issn.1674-4500.2025.05.05
超声造影灌注缺损特征联合EMT基因与血管生成调控网络预测乳腺浸润性导管癌侵袭性
Prediction of invasiveness of invasive ductal carcinoma of the breast using contrast-enhanced ultrasound perfusion defect characteristics combined with EMT gene and angiogenesis regulatory network
摘要
Abstract
Objective To explore the relationship and clinical significance between ultrasound characteristics and the expression of epithelial mesenchymal transition(EMT)genes in breast invasive ductal carcinoma.Methods A literature search was conducted in the PubMed database,utilizing the National Center of Biotechnology Information and Gene Expression Profiting Interactive Analysis for comprehensive analysis to identify pertinent EMT genes.Gather information from 38 individuals identified with invasive ductal carcinoma of the breast through pathology at Shanxi Medical University's First Hospital from September 2022 to August 2023,encompassing clinical symptoms and ultrasound characteristics,to investigate their connection with EMT gene expression.Results Nine EMT genes were chosen in total.Notably,the expression level of VIM in patients over 50 years old or with lymph node metastasis was significantly higher than that in the control group(P<0.05).Additionally,the expression levels of the EMT genes VIM,MMP2,ZEB1,and ZEB2 were negatively correlated with PI(r=-0.500,-0.424,-0.548,-0.561,P<0.05),and similarly,these genes were also negatively correlated with AUC(r=-0.525,-0.470,-0.464,-0.522,P<0.05).Conversely,the expression levels of ZEB1 and ZEB2 exhibited positive correlations with AT(r=0.329,0.403,P<0.05)and TTP(r=0.336,0.389,P<0.05),respectively.Furthermore,compared to the uniform contrast perfusion group,the expression levels of VIM,MMP2,MMP9,and ZEB2 were elevated in the uneven contrast perfusion group.In addition,the levels of VIM,MMP2,ZEB1,and ZEB2 significantly increased in the group with a perfusion defect area greater>1/2,compared to the group with a perfusion defect area less≤1/2.Among these genes,VIM,MMP2,and ZEB2 showed significant correlations with one another.Conclusion A link exists between the ultrasound features of breast invasive ductal carcinoma and the EMT gene,offering imaging proof for clinical diagnosis and therapy,reflecting the angiogenesis and hypoxic necrosis status within the lesion,and EMT could be exploited as a potential therapeutic target to inhibit tumor progression.关键词
乳腺癌/上皮间质转化/超声造影/血管生成/基因Key words
breast cancer/epithelial mesenchymal transformation/contrast-enhanced ultrasound/angiogenesis/gene引用本文复制引用
李欣榕,任家仪,胡道秀,曹静玮,陈耀东,陈武..超声造影灌注缺损特征联合EMT基因与血管生成调控网络预测乳腺浸润性导管癌侵袭性[J].分子影像学杂志,2025,48(5):562-568,7.基金项目
山西省自然科学研究面上项目(20210302123259) （20210302123259）
