中国妇幼健康研究2025，Vol.36Issue(5)：16-23,8.DOI:10.3969/j.issn.1673-5293.2025.05.003
MIF缺失抑制子宫内膜癌细胞的增殖转移及机制研究
Study on the inhibitory effect and mechanism of MIF deficiency on the proliferation and metastasis of endometrial cancer cells
摘要
Abstract
Objective To investigate the effects and regulatory mechanisms of macrophage migration inhibition factor(MIF)deficiency on metastasis and ferroptosis in endometrial cancer(EC)cells.Methods Western blot analysis was used to detect MIF expression levels in human endometrial endothelial cells(hEEC)and EC cell lines(Ishikawa,KLE,RL95-2 and AN3-CA).Ishikawa and KLE cells were divided into four groups.si-NC transfection group(si-NC),si-MIF transfection group(si-MIF),si-MIF+oe-NC transfection group(si-MIF+oe-NC),and si-MIF+oe-SLC7A1 transfection group(si-MIF+oe-SLC7A1).Western blotting was used to measure the levels of MIF,SLC7A1,p-AKT/AKT,and p-GSK-3β/GSK-3β in each group.Cell viability was assessed using the CCK-8 assay,while wound healing and Transwell assays were performed to evaluate cell migration and invasion,respectively.Additionally,malondialdehyde(MDA)and Fe2+levels were measured.Results Compared to the hEEC group,MIF expression was significantly upregulated in Ishikawa,KLE,RL95-2,and AN3-CA cells(F=23.933,P＜0.001).Compared to the si-NC group,the si-MIF and si-MIF+oe-NC groups exhibited significantly reduced SLC7A1 expression(F=20.157,P＜0.001).Furthermore,p-AKT/AKT and p-GSK-3β/GSK-3β levels,cell proliferation,migration,and invasion abilities were significantly decreased in the si-MIF and si-MIF+oe-NC groups(F=12.456,18.234,32.451,12.257 and 15.364,respectively,P＜0.001),while MDA and Fe2+levels were significantly increased(F=40.215 and 38.845,respectively,P＜0.001).Compared to the si-MIF+oe-NC group,the si-MIF+oe-SLC7A1 group showed significantly increased p-AKT/AKT and p-GSK-3β/GSK-3β levels,as well as enhanced cell proliferation,migration,and invasion abilities(F=12.397,21.045,26.229,18.330 and 14.228,respectively,P＜0.001),while MDA and Fe2+levels were significantly decreased(F=25.294 and 26.005,respectively,P＜0.001).Conclusion MIF deficiency may inhibit the AKT/GSK-3β signaling pathway by suppressing SLC7A1 expression,thereby promoting ferroptosis and inhibiting EC cell proliferation,migration,and invasion.关键词
子宫内膜癌/巨噬细胞迁移抑制因子/信号通路/细胞转移/铁死亡Key words
endometrial cancer/macrophage migration inhibition factor/signaling pathway/cell migration/ferroptosis分类
预防医学引用本文复制引用
崔志利,安欣,刘文利,李静霞..MIF缺失抑制子宫内膜癌细胞的增殖转移及机制研究[J].中国妇幼健康研究,2025,36(5):16-23,8.基金项目
邯郸市科学技术研究与发展计划(19422083029ZC) （19422083029ZC）
