临床与病理杂志2025，Vol.45Issue(2)：168-176,9.DOI:10.11817/j.issn.2095-6959.2025.240980
嗜酸性粒细胞性肠炎患儿小肠黏膜ILC3数量和IL-22表达的变化及其临床意义
Changes in intestinal mucosal ILC3 counts and IL-22 expression in children with eosinophilic enteritis and their clinical significance
王丽媛 1吴艳玲 1汪陈慧 1郑新国 1胡艳翎 1沈梦雨 1杨辉1
作者信息
- 1. 南京医科大学附属儿童医院消化科,南京 210008
- 折叠
摘要
Abstract
Objective:The pathogenesis of eosinophilic enteritis(EoN)is complex and involves multiple cells and molecules,but remains incompletely understood.This study aims to investigate the changes in intestinal mucosal type 3 innate lymphoid cells(ILC3)and interleukin-22(IL-22)expression in children with EoN and assess their clinical significance.
Methods:A total of 20 children diagnosed with EoN between June 2020 and December 2021 were included in the EoN group.Another 20 children presenting with abdominal pain,hematochezia,or other symptoms but excluded from EoN diagnosis served as the control group.Peripheral blood eosinophil(EOS)counts and immunoglobulin E(IgE)levels were measured.HE staining was used to evaluate EOS infiltration in duodenal and ileal mucosa.Immunohistochemical staining was performed to detect IL-22 and ZO-1 protein expression,while dual immunohistochemical staining was used to quantity ILC3s.Real-time RT-PCR was conducted to assess IL-22 and ZO-1 mRNA expression.Correlations between ILC3 counts,IL-22 levels,EOS counts,and ZO-1 levels in the mucosa were analyzed.
Results:Peripheral EOS counts and total IgE levels in the EoN group were significantly higher than those in the control group(both P<0.05).EOS infiltration in the duodenal and ileal mucosa was significantly increased in the EoN group(P<0.05).The number of ILC3s and the levels of IL-22 in both duodenal and ileal mucosa were significantly reduced in EoN patients compared to controls.These were negatively correlated with EOS infiltration and positively correlated with ZO-1 levels(P<0.05).
Conclusion:Reduced intestinal mucosal ILC3 counts and IL-22 expression,as well as impaired mucosal barrier integrity,may contribute to the pathogenesis of eosinophilic enteritis in children.关键词
嗜酸粒细胞性肠炎/3型固有淋巴细胞/白细胞介素22/嗜酸性粒细胞/儿童Key words
eosinophilic enteritis/type 3 innate lymphoid cells/interleukin-22/eosinophils/children引用本文复制引用
王丽媛,吴艳玲,汪陈慧,郑新国,胡艳翎,沈梦雨,杨辉..嗜酸性粒细胞性肠炎患儿小肠黏膜ILC3数量和IL-22表达的变化及其临床意义[J].临床与病理杂志,2025,45(2):168-176,9.
