临床与病理杂志2025，Vol.45Issue(2)：177-185,9.DOI:10.11817/j.issn.2095-6959.2025.240997
川崎病患儿CAT血清浓度的变化及其基因多态性与川崎病易感性的关系
Changes in serum CAT level and the association of CAT gene polymorphisms with susceptibility to Kawasaki disease
摘要
Abstract
Objective:Kawasaki disease(KD)is the most common autoimmune vasculitis syndrome in children.Catalase(CAT),one of the most crucial antioxidant enzymes in the human body,plays a crucial role in maintaining free radical balance.This study aims to investigate changes in serum CAT level in children with KD and the relationship of CAT gene polymorphisms with KD susceptibility. Methods:Serum CAT concentrations were measured by enzyme-linked immunosorbent assay(ELISA)in 27 children in the acute phase of KD(acute group),27 in the convalescent phase(convalescent group),and 26 healthy children(control group).Additionally,108 KD patients and 115 healthy children were selected for genotyping of three CAT gene loci(rs1001179,rs7943316,and rs1049982)using polymerase chain reaction(PCR)and Sanger sequencing. Results:1)Serum CAT concentrations differed significantly among the acute KD group,the convalescent KD group,and the control group(all P<0.01).CAT level was significantly lower in the acute group compared to both the convalescent group and the control group(both P<0.01).At a serum CAT level of 8.55×104 pg/mL,the sensitivity and specificity for diagnosing acute KD were 63.00%and 88.50%,respectively,with an area under the receiver operating characteristic curve of 0.758,indicating high diagnostic value.2)There were no statistically significant differences in the genotype or allele frequencies of rs1001179,rs7943316,and rs1049982 between the KD and control groups(P>0.05).Similarly,no significant differences were found between KD patients with and without coronary artery lesions in terms of genotype or allele distribution(P>0.05). Conclusion:Serum CAT level decreases significantly during the acute phase of KD,suggesting CAT is involved in the pathogenesis of the disease.However,the polymorphisms at CAT gene loci rs1001179,rs7943316,and rs1049982 are not significantly associated with KD susceptibility or the presence of coronary artery lesions.关键词
川崎病/过氧化氢酶/基因多态性/冠状动脉损伤/氧化应激Key words
Kawasaki disease/catalase/genetic polymorphism/coronary artery lesion/oxidative stress引用本文复制引用
吴吉平,董杨,李旭,申祥宇,李卓颖,杨作成,陈敬华,张志宏..川崎病患儿CAT血清浓度的变化及其基因多态性与川崎病易感性的关系[J].临床与病理杂志,2025,45(2):177-185,9.基金项目
国家自然科学基金(81800440,82204697) （81800440,82204697）
吉林省卫生健康科技能力提升项目(2023JC030) （2023JC030）
吉林省大学生创新创业训练计划项目(省级项目)(202410201085) （省级项目）
北华大学博士科研启动基金(20220038).This work was supported by the National Natural Science Foundation(81800440,82204697),the Jilin Province Health Science and Technology Capability Enhancement Project(2023JC0300),the Jilin Province College Students'Innovation and Entrepreneurship Training Program(Provincial Project)(202410201085),and the Beihua University Doctoral Research Start up Fund(20220038),China. （20220038）
