海南医科大学学报2025，Vol.31Issue(10)：732-738,7.DOI:10.13210/j.cnki.jhmu.20241011.002
黄皮提取物对白假丝酵母菌及其生物膜的抑制作用研究
Study on the inhibitory effect of Clausena lansium(Lour.)Skeels extract on Candida albicans and its biofilm
摘要
Abstract
Objective:To investigate the antifungal effect of extract from Clausena lansium(Lour.)Skeels on Candida albi-cans(Ca)and its mechanism of biofilm inhibition.Methods:The minimum inhibitory concentrations(MICs)and minimum fungal concentrations(MFCs)of extracts from various parts of Clausena lansium against the standard strain Ca SC5314 and the resistant strain Ca N56 were determined by micro dilution method.The growth curves and the inhibitory effects were determined using the time growth curve method and XTT method,respectively.Using a microscope to observe the effect of Clausena lansium extract on the integrity of Ca biofilm and using RT-qPCR technology to detect the expression of als3,efg1,hwp1,and eap1 genes.Re-sults:The seed extract of Clausena lansium had the best antifungal effect on Ca with MICs at 0.50～1.00 mg/mL and MFCs at 1.00～2.00 mg/mL.The antifungal effect on Ca N56 resistant strains was better than that of Ca SC5314.Clausena lansium seed extract had a certain inhibitory effect on the formation of biofilm,and the greater the concentration,the more obvious the inhibitory effect.Clausena lansium extract could downregulate the expression of biofilm related genes als3,efg1,hwp1,and eap1.Conclu-sion:Clausena lansium extract may inhibit the formation and integrity of Ca biofilm by downregulating the expression of biofilm re-lated genes als3,efg1,hwp1,and eap1,ultimately leading to its inhibitory effect on Ca.It also provides a reference for the natural and effective drugs for clinical treatment of serious drug resistant Ca.关键词
黄皮提取物/白假丝酵母菌/生物膜/耐药/基因表达Key words
Clausena lansium extract/Candida albicans/Biofilm/Drug resistance/Gene expression分类
中医学引用本文复制引用
王岩,黄丽玲,柴金萱,马寅正,易国辉,吴金燕,吴紫贝,陈琼花,何小稳..黄皮提取物对白假丝酵母菌及其生物膜的抑制作用研究[J].海南医科大学学报,2025,31(10):732-738,7.基金项目
This study was supported by the Natural Science Foundation Project of Hainan Province(822MS077),Scientific Research Projects of Higher Education Institutions of Hainan(Hnky2023-22,Hnky2024-24),and Innovation and Entrepreneurship Training Program for College Students of Hainan Medical University(X202311810124) 海南省自然科学基金资助项目(822MS077) （822MS077）
海南省高等学校科学研究项目(Hnky2023-22、Hnky2024-24) （Hnky2023-22、Hnky2024-24）
海南医学院大学生创新创业训练计划项目(X202311810124) （X202311810124）
