海南医科大学学报2025，Vol.31Issue(10)：739-749,11.DOI:10.13210/j.cnki.jhmu.20250114.002
基于NT-3/TrkC信号通路探讨电针对血管性痴呆大鼠海马突触可塑性及炎症因子的影响
Exploration of electroacupuncture on synaptic plasticity and inflammatory factors in the hippocampus of vascular dementia rats based on the NT-3/TrkC signaling pathway
摘要
Abstract
Objective:To investigate the possible mechanism of electroacupuncture"Sishencong"and"Fengchi"on protecting and improving hippocampal synaptic plasticity and inhibiting inflammation in rats with vascular dementia based on the NT-3(neu-rotrophic factor 3)/TrkC(tyrosine kinase receptors C)signaling pathway.Methods:The 60 SD rats after the Morris water laby-rinth test were randomly divided into the blank groups,the fake surgery groups,and the surgery groups.The four blood vessel blocking method(4-VO method)made the mouse model of the surgical group,and the 24 rats that were successfully made were randomly divided into the model group and the electronic needle group.Seven days after the molding of the electric needle group rats were taken 21 consecutive days of electro-needle intervention was done,the left and right"Sishencong"and the two-sided"Fengchi"acupoints were taken,30 minutes each time,once a day.Before the molding,after the molding,and the intervention,the Morris water maze test was used to detect the learning and cognitive ability of rats;the HE staining method was used to detect the changes in the pathological morphology of rats and hippocampus;the immunohistochemical method(IHC)detected the NL-RP3,NT-3,small gel cell IBA-1(ionic calcium connector protein)positive expression in the hippocampus;enzyme linked immu-nosorbent assay(ELISA)was applied to measure hippocampus tissue leukocyte-1β(IL-1β)and leukocytes-18(IL-18)content;Western blot was performed to determine hippocampus NT-3,TRKC,NLRP3,Caspase-1(caspase-1),SYN(synaptophysin)and PSD95(postsynaptic dense 95)protein expression.Results:The results of the water maze test showed that compared with the blank group and the fake surgery group,the model group's rats showed increased escape latency and the number of successful crossing platforms decreased(P<0.05);compared with the model group,the electronic needle group rats exhibited decreased es-cape latency and increased number of crossing(P<0.05);compared with the blank group and the fake surgery group,the IHC showed Iba-1 and NLRP3 expressions were heighted significantly(P<0.01),NT-3 significantly reduced in the model group(P<0.01);in the mice of the electrical needle group,the shape of small gel cells recovered,and the expression of Iba-1 and NLRP3 was decreased(P<0.05,P<0.01),NT-3 expression was increased compared with the model group(P<0.01);ELISA results showed that the contents of IL-1β and IL-18 in hippocampus of model group were significantly increased compared with the blank group and the fake surgery group(P<0.01);these contents were decreased in the electronic needle group(P<0.05);western blot detection showed that compared with the blank group and the fake surgery group,Heman NT-3,TRKC,PSD95,SYN pro-tein expression was significantly reduced in the model group(P<0.05,P<0.01),NLRP3 and Caspase-1 protein expression signif-icantly increased(P<0.05,P<0.01);compared with the model group NT-3,TRKC,PSD95 and SYN protein expressions were markedly upregulated(P<0.05,P<0.01)and NLRP3 and Caspase-1 protein expressions were significantly reduced in the elec-tronic needle group(P<0.05,P<0.01).Conclusion:Electroacupuncture may increase the expression of NT-3 in the hippocam-pus of vascular dementia rats,promote the binding of NT-3 to TrkC,reduce the expression of NLRP3 protein,alleviate microglia-induced neuroinflammation,improve synaptic plasticity,thus have a protective effect on the brain,and improve learning and memory ability.关键词
电针/血管性痴呆/神经营养因子3(NT-3)/NOD样受体热蛋白结构域3(NLRP3)/突触可塑性Key words
Electroacupuncture/Vascular dementia/Neurotrophic factor 3(NT-3)/NOD-like receptor thermoprotein struc-tural domain 3(NLRP3)/Synaptic plasticity分类
中医学引用本文复制引用
王浩宇,韩玉生,李欣然,苗悦,陈英华,孙玮,宋元毓,秦瑞琦,李俊峰,苏晓庆,吴彤,赵红旭..基于NT-3/TrkC信号通路探讨电针对血管性痴呆大鼠海马突触可塑性及炎症因子的影响[J].海南医科大学学报,2025,31(10):739-749,11.基金项目
国家自然科学基金项目(82074530、82474650) （82074530、82474650）
黑龙江省自然科学基金项目(ZD2021H007) （ZD2021H007）
黑龙江省中医药科研项目(ZHY2023-189、ZHY2023-209) （ZHY2023-189、ZHY2023-209）
黑龙江中医药大学研究生创新科研项目(2024yjscx001) This study was supported by National Natural Science Foundation of China(82074530,82474650) （2024yjscx001）
Natural Science Foundation of Heilongjiang Province(ZD2021H007) （ZD2021H007）
Chinese Medicine Research Project of Heilongjiang Province(ZHY2023-189,ZHY2023-209) （ZHY2023-189,ZHY2023-209）
Graduate Innovative Research Project of Heilongjiang University of Chinese Medicine(2024yjscx001) （2024yjscx001）
