湖南中医药大学学报2025，Vol.45Issue(5)：787-794,8.DOI:10.3969/j.issn.1674-070X.2025.05.002
基于"以方测证"理论探讨腹主动脉缩窄术致慢性心力衰竭大鼠模型的中医证型
Exploring the TCM pattern in the rat model of chronic heart failure induced by abdominal aortic constriction based on the theory of"pattern identification by formula"
摘要
Abstract
Objective To observe the therapeutic differences of four TCM injections on the rat model of chronic heart failure(CHF)induced by abdominal aortic constriction(AAC),and to explore the TCM pattern attributes of the model based on the theory of"pattern identification by formula".Methods AAC was performed on 100 SD rats,with additional 15 rats assigned to a sham-operation group.Eight weeks after modeling,the successful establishment of the CHF rat model was verified.A total of 68 AAC rats with successful modeling were randomly divided into model group,Danhong Injection group,Shenmai Injection group,Shenfu Injection group,Huangqi Injection group,and captopril group.The Danhong,Shenmai,Shenfu,and Huangqi Injection groups were intraperitoneally injected with the corresponding injections of 6 mL/kg,along with intragastric administration of saline at 4 mL/kg for intervention.The captopril group received daily intragastric administration of captopril at 8.8 mg/kg,combined with intraperitoneal injection of saline 6 mL/kg for intervention.Both the sham-operation and model groups received equivalent volumes of saline via intraperitoneal injection and intragastric administration.Each group underwent a 15-day intervention period.After the administration period,the general conditions of the rats in each group were observed.Echocardiography was used to assess cardiac function.ELISA was employed to determine serum levels of N-terminal pro-brain natriuretic peptide(NT-proBNP),angiotensin Ⅱ(Ang Ⅱ),and angiotensin type 1 receptor(AT1R).HE staining and Masson's trichrome staining were both utilized to examine pathological changes in myocardial tissue.Results Compared with the sham-operation group,the model group rats exhibited dull skin and hair,cyanosis of the lips,nails,and abdomen,hair loss,irritability,and reduced activity.Left ventricular ejection fraction(LVEF)and left ventricular fractional shortening(LVFS)significantly decreased(P＜0.01),while left ventricular internal diameter at end-diastole(LVIDd),left ventricular internal diameter at end-systole(LVIDs),serum levels of NT-proBNP,Ang Ⅱ,and AT1R,as well as collagen volume fraction(CVF)significantly elevated(P＜0.01),with prominent myocardial inflammatory infiltration.In comparison to the model group,the Danhong Shenmai,Shenfu,Huangqi Injection groups and captopril group demonstrated improved responsiveness,enhanced skin luster,and ameliorated cyanosis of the lips,nails,and abdomen.These groups exhibited increased LVEF and LVFS values(P＜0.05,P＜0.01),while LVIDd,LVIDs,serum NT-proBNP,Ang Ⅱ,AT1R levels,and CVF(P＜0.05,P＜0.01)decreased.with alleviated myocardial inflammatory infiltration.Compared with the Danhong Injection group,the Shenmai,Shenfu,and Huangqi Injection groups showed decreased LVEF values(P＜0.05)and increased LVIDd,LVIDs,serum NT-proBNP,Ang Ⅱ,AT1R levels,and CVF(P＜0.05,P＜0.01),with more severe myocardial inflammatory infiltration.Conclusion The Danhong Injection effectively improves cardiac function,reduces inflammatory responses,and inhibits excessive neuroendocrine activation in the AAC-induced CHF rat model.Its therapeutic efficacy is superior to the other three injections.Based on the theory of"pattern identification by formula",it is hypothesized that the TCM pattern of this model corresponds to heart blood stasis pattern.关键词
腹主动脉缩窄术/慢性心力衰竭/以方测证/丹红注射液/参麦注射液/参附注射液/黄芪注射液Key words
abdominal aortic constriction/chronic heart failure/pattern identification by formula/Danhong Injection/Shenmai Injection/Shenfu Injection/Huangqi Injection分类
中医学引用本文复制引用
叶嘉豪,吴子政,张垚,孟骊冲,胡志希..基于"以方测证"理论探讨腹主动脉缩窄术致慢性心力衰竭大鼠模型的中医证型[J].湖南中医药大学学报,2025,45(5):787-794,8.基金项目
国家自然科学基金面上项目(82274412) （82274412）
湖南中医药大学校级研究生创新课题项目(2023CX01). （2023CX01）
