湖南中医药大学学报2025，Vol.45Issue(5)：795-802,8.DOI:10.3969/j.issn.1674-070X.2025.05.003
加减麦门冬汤对小鼠急性肺损伤及TLR4/MyD88/NF-κB p65信号通路的影响
Effects of modified Maimendong Decoction on acute lung injury in mice and the TLR4/MyD88/NF-κB p65 signaling pathway
摘要
Abstract
Objective To investigate the effects and mechanisms of modified Maimendong Decoction(MMD)on lipopolysaccharide(LPS)-induced acute lung injury(ALI)in mice.Methods Forty-two BALB/c mice were randomly divided into six groups with 7 mice in each group:control group,model group(LPS 5 mg/kg),dexamethasone group(5 mg/kg),and low-(7.5 g/kg),medium-(15 g/kg),and high-dose(30 g/kg)MMD groups.LPS was used to induce ALI in mice.The lung wet/dry(W/D)ratio was measured to assess pulmonary edema.HE staining was employed to observe histopathological changes in lung tissues.ELISA was used to detect serum levels of inflammatory cytokines,including interleukin(IL)-1 β,IL-6,and tumor necrosis factor-α(TNF-α).Colorimetric assays were performed to evaluate oxidative stress-related indicators in lung tissues,such as expression levels of malondialdehyde(MDA),nitric oxide(NO),and total superoxide dismutase(T-SOD).Western blot was conducted to determine the protein expression levels of TLR4,MyD88),NF-κB p65,and p-NF-κB p65 in the Toll-like receptor 4(TLR4)/myeloid differentiation factor 88(MyD88)/nuclear transcription factor-κB p65(NF-κB p65)signaling pathway.Results Compared with the control group,the model group exhibited aggravated pulmonary hemorrhage,edema,and inflammatory cell infiltration.Serum levels of inflammatory cytokines IL-1β,IL-6,and TNF-α significantly elevated(P＜0.01).Lung tissue levels of NO and MDA,as well as protein expression levels of TLR4,MyD88,and p-NF-κB p65/NF-κB p65 markedly increased(P＜0.01),while T-SOD expression level decreased(P＜0.01).In comparison to the model group,the dexamethasone group and the medium-and high-dose MMD groups showed relatively reduced pulmonary hemorrhage,edema,and inflammatory cell infiltration.Serum expression levels of IL-1β,IL-6,and TNF-α were significantly lower(P＜0.01),and lung tissue levels of NO and MDA,along with TLR4,MyD88,and p-NF-κB p65/NF-κB p65 protein expression levels,decreased(P＜0.01 or P＜0.05),whereas T-SOD expression level elevated(P＜0.01).Compared with the low-dose MMD group,the medium-and high-dose MMD groups demonstrated further attenuation of pulmonary hemorrhage,edema,and inflammation.The high-dose MMD group showed markedly reduced serum expression levels of IL-1β,IL-6,and TNF-α(P＜0.01),as well as decreased lung tissue expression levels of NO,MDA,and p-NF-κB p65/NF-κB p65 proteins(P＜0.01),accompanied by increased T-SOD expression levels(P＜0.01).Conclusion MMD exhibits protective effects against ALI in mice,likely through modulating the TLR4/MyD88/NF-κB p65 signaling pathway to attenuate inflammatory responses.关键词
急性肺损伤/加减麦门冬汤/脂多糖/炎症/TLR4/MyD88/NF-κB p65信号通路Key words
acute lung injury/modified Maimendong Decoction(MMD)/lipopolysaccharide/inflammation/TLR4/MyD88/NF-κB p65 signaling pathway分类
中医学引用本文复制引用
张冉阳,欧阳倪冰,王鹏,周芳亮,高强..加减麦门冬汤对小鼠急性肺损伤及TLR4/MyD88/NF-κB p65信号通路的影响[J].湖南中医药大学学报,2025,45(5):795-802,8.基金项目
国家自然科学基金项目(82104941) （82104941）
中西医结合病原生物学湖南省重点实验室开放基金(2022KFJJ06) （2022KFJJ06）
湖南中医药大学2023年大学生创新创业训练计划(湘教通[2023]132号)第224号 （湘教通[2023]132号）
湖南中医药大学中西医结合一流学科开放基金(2020ZXYJH03,2020ZXYJH73) （2020ZXYJH03,2020ZXYJH73）
湖南中医药大学一流学科《基础医学》(NO:1) （NO:1）
湖南中医药大学重点培育学科基础医学学科(校行发规字[2023]2号)资助. （校行发规字[2023]2号）
