湖南中医药大学学报2025，Vol.45Issue(5)：803-809,7.DOI:10.3969/j.issn.1674-070X.2025.05.004
芎芪方调控氧化三甲胺对心肌缺血再灌注损伤的影响
Effects of Xiongqi Formula on myocardial ischemia-reperfusion injury by regulating trimethylamine N-oxide
摘要
Abstract
Objective To explore the mechanism of action of Xiongqi Formula(XQF)in regulating trimethylamine N-oxide(TMAO)to protect against myocardial ischemia-reperfusion injury(MIRI)based on"the heart and small intestine being exteriorly and interiorly related to each other".Methods Fifty male Sprague-Dawley rats were randomly divided into five groups(10 rats per group):sham-operated,model,XQF 3,3-dimethyl-1-butanol(DMB),and XQF+DMB.The sham-operated and model groups received an equivalent volume of saline.All groups underwent intragastric administration for 14 consecutive days.The MIRI model was established by ligating the rat left anterior descending coronary artery.After modeling,blood was collected from the rat abdominal aorta.HE staining was used to observe histopathological changes in the rat myocardial and jejunal tissues.ELISA was used to determine serum creatine kinase isoenzyme MB(CK-MB),lactate dehydrogenase(LDH),malondialdehyde(MDA),superoxide dismutase(SOD),interleukin(IL)-1 β,and IL-6 in rats.Triple quadrupole liquid chromatography-mass spectrometry was used to measure serum TMAO levels in rats.Results Compared with the sham-operated group,the rat myocardial and jejunal tissues in the model group showed pathological damage such as myocardial fiber rupture,inflammatory infiltration,and jejunal villus swelling and rupture.The serum levels of CK-MB,LDH,MDA,IL-1 β,IL-6,and TMAO significantly increased(P＜0.05),while the SOD activity decreased(P＜0.05).Compared with the model group,the XQF,DMB,and XQF+DMB groups showed attenuated myocardial and jejunal damage,with reduced serum CK-MB,LDH,MDA,IL-1β,IL-6,and TMAO levels(P＜0.05)and increased SOD activity(P＜0.05).Compared with the XQF group,the DMB group displayed more severe myocardial fiber rupture,cardiomyocyte deformation,vacuolization,elevated serum CK-MB,LDH,MDA,IL-1β,and TMAO levels(P＜0.05),and reduced SOD activity(P＜0.05).The XQF+DMB group showed mild myocardial cell dissolution,slight inflammatory infiltration,widened intercellular spaces,and increased CK-MB,LDH,MDA,and IL-1β levels(P＜0.05).Compared with the DMB group,myocardial fiber dissolution and inflammatory infiltration in the XQF+DMB group were alleviated but jejunal villus swelling was aggravated;the serum CK-MB,LDH,MDA,IL-1β,IL-6,and TMAO levels decreased(P＜0.05),while the SOD activity increased(P＜0.05).Conclusion"The heart and small intestine being exteriorly and interiorly related to each other"is an important scientific basis for modern medicine to treat MIRI from the perspective of the intestine.XQF may exert a preventive protective effect against myocardial ischemia-reperfusion in MIRI stress events by regulating the TMAO-mediated inflammatory signaling pathway and thereby alleviating oxidative stress and inflammatory cascade.关键词
心肌缺血再灌注损伤/心与小肠相表里/氧化三甲胺/川芎/黄芪Key words
myocardial ischemia-reperfusion injury/the heart and small intestine being exteriorly and interiorly related to each other/trimethylamine N-oxide/Chuanxiong(Chuanxiong Rhizoma)/Huangqi(Astragali Radix)分类
中医学引用本文复制引用
覃浩昌,杨漾,张慧卿,郑淋淋,李扬,余嗣澳,陈聪,廖菁..芎芪方调控氧化三甲胺对心肌缺血再灌注损伤的影响[J].湖南中医药大学学报,2025,45(5):803-809,7.基金项目
国家自然科学基金项目(81704065) （81704065）
湖南省自然科学基金项目(2023JJ30455,2025JJ60804) （2023JJ30455,2025JJ60804）
湖南省教育厅科学研究项目(22A0274,23C0172) （22A0274,23C0172）
湖南省中医药科研计划项目(A2023030,B2023022) （A2023030,B2023022）
湖南中医药大学研究生创新项目(2023CX172) （2023CX172）
湖南中医药大学平台开放基金项目(2022FPKFJJ04). （2022FPKFJJ04）
