湖南中医药大学学报2025，Vol.45Issue(5)：810-817,8.DOI:10.3969/j.issn.1674-070X.2025.05.005
矾冰纳米乳调节Wnt/β-catenin通路对糖尿病型肛瘘术后创面愈合的影响
Effects of alum-ice nanoemulsion on wound healing after surgery for diabetic anal fistula by regulating Wnt/β-Catenin pathway
摘要
Abstract
Objective To observe the effects of alum-ice nanoemulsion on postoperative wound healing in diabetic rats with anal fistula and the expression of proteins related to the Wnt/β-catenin signaling pathway in rats after surgery for diabetic anal fistula,and to explore its mechanism of action in promoting wound repair.Methods Seventy-two SPF-grade female SD rats were randomly divided into sham-operated group,model group,alum-ice nanoemulsion group,and Moist Burn Ointment(MBO)group,with 18 rats in each group.A diabetic rat model was established in all groups.Subsequently,a postoperative wound model of diabetic anal fistula was created in all groups except the sham-operated group.The model group received daily local coverage with saline-soaked gauze,the nanoemulsion group received alum-ice nanoemulsion-soaked gauze,and the MBO group received MBO-soaked gauze.The treatments were applied for 21 consecutive days.The body weight,mental status,activity ability,and wound healing rate of the rats in each group were observed on the 7th,14th,and 21st days respectively.HE staining was used to examine the pathological changes and capillary density in the wound tissue.Western blot was employed to check the expression levels of Wnt1,β-catenin,Cyclin D1,C-myc,vascular endothelial growth factor(VEGF),vascular endothelial growth factor receptor 2(VEGFR2),and glycogen synthase kinase 3β(GSK-3β)proteins in wound tissue.Results Compared with the sham-operated group,rats in the model group showed decreased body weight and wound healing rate(P＜0.05),increased inflammatory cell infiltration,and reduced capillary count in the wound tissue(P＜0.05).On day 21,the expression levels of Wnt1,β-catenin,Cyclin D1,and C-myc proteins were significantly elevated(P＜0.05,P＜0.01),while the expression levels of VEGF,VEGFR2,and GSK-3β significantly decreased(P＜0.01).Compared with the model group,both the alum-ice nanoemulsion group and the MBO group showed increased body weight and wound healing rate(P＜0.05),reduced inflammatory cell infiltration,and increased capillary count(P＜0.05).On day 21,the expression levels of Wnt1,β-catenin,Cyclin D1,C-myc,VEGF,and VEGFR2 proteins were significantly upregulated(P＜0.05,P＜0.01),while GSK-3β expression was significantly downregulated(P＜0.01).Conclusion Alum-ice nanoemulsion may promote wound healing by activating proteins related to the Wnt/β-catenin signaling pathway,thereby improving the histopathological condition of the wound tissue.关键词
矾冰纳米乳/Wnt/β-catenin信号通路/糖尿病/肛瘘/术后创面Key words
alum-ice nanoemulsion/Wnt/β-catenin signaling pathway/diabetes/anal fistula/postoperative wound分类
中医学引用本文复制引用
陆文洪,刘丽芳,王真权,罗雯鹏..矾冰纳米乳调节Wnt/β-catenin通路对糖尿病型肛瘘术后创面愈合的影响[J].湖南中医药大学学报,2025,45(5):810-817,8.基金项目
湖南省中医药管理局一般资助项目(B2023149) （B2023149）
湖南省卫生健康委员会卫生科研课题一般资助项目(B202304136861) （B202304136861）
湖南省教育厅优秀青年项目(24B0360) （24B0360）
湖南中医药大学研究生创新课题(2024CX009) （2024CX009）
湖南中医药大学校院联合基金教学改革研究重点项目(2022-LHJG003). （2022-LHJG003）
