湖南中医药大学学报2025，Vol.45Issue(5)：862-868,7.DOI:10.3969/j.issn.1674-070X.2025.05.011
电针对脊髓损伤大鼠肠道动力及直肠Ca2+/CaM/MLCK信号通路的影响
Effects of electro-acupuncture on intestinal motility and rectal Ca2+/CaM/MLCK signaling pathway in rats with spinal cord injury
摘要
Abstract
Objective To observe the effects of electro-acupuncture(EA)on calcium ion(Ca2+),calmodulin(CaM),and myosin light-chain kinase(MLCK)in rectal tissue of rats with spinal cord injury(SCI),so as to explore the potential mechanism of EA in improving intestinal motility of SCI rats.Methods Among 42 adult female SD rats,ten were randomly selected as the sham-operated group,while the remaining 32 rats underwent complete transection of the spinal cord at the T10 spinal segment to establish a SCI model.Twenty successfully modeled rats were randomly divided into model group and EA group,with ten rats in each group.The EA group was given EA intervention starting from day 19 after modeling,20 minutes per session,once a day,for seven consecutive days.The sham-operated and model groups underwent only restraint without intervention.The general condition,body weight change,fecal water content,and intestinal propulsion rate of rats were observed before and after treatment in groups.The contractility of rectal smooth muscle was observed by in vitro rectal smooth muscle contraction test.The Ca2+concentration of rectal tissue was determined by colorimetry,and the expression levels of CaM and MLCK proteins in rectal smooth muscle tissue were checked by Western blot.Results After intervention,compared with the sham-operated group,the model group exhibited lethargy,poor mental state,reduced food and water intake,body weight,fecal water content,and intestinal propulsion rate(P＜0.05).Spontaneous contractions of isolated rectal smooth muscle strips showed reduced maximal/minimal tension,amplitude,and frequency(P＜0.05).The Ca2+concentration in rectal tissue(P＜0.05)and the CaM and MLCK protein expression levels in rectal smooth muscle tissue decreased(P＜0.05).Compared with the model group,the EA group showed improved mental state,increased food and water intake,and elevated body weight,fecal water content,and intestinal propulsion rate(P＜0.05).Spontaneous contractions of rectal smooth muscle strips demonstrated enhanced maximal/minimal tension,amplitude,and frequency(P＜0.05),along with increased rectal tissue Ca2+concentration(P＜0.05)and CaM/MLCK protein expression levels(P＜0.05).Conclusion EA can improve intestinal motility in SCI rats and its mechanism may be related to the regulation of Ca2+/Ca M/ML C K signaling pathway.关键词
脊髓损伤/肠道动力/电针/钙离子/钙调蛋白/肌球蛋白轻链激酶Key words
spinal cord injury/intestinal motility/electro-acupuncture/calcium ion/calmodulin/myosin light-chain kinase分类
医药卫生引用本文复制引用
罗小精,田楚宁,展立芬,李芊,梁柔筠,林志坚,卓越,许明,张泓..电针对脊髓损伤大鼠肠道动力及直肠Ca2+/CaM/MLCK信号通路的影响[J].湖南中医药大学学报,2025,45(5):862-868,7.基金项目
国家自然科学基金面上项目(82274666) （82274666）
湖南中医药大学学科建设"揭榜挂帅"项目(22JBZ013) （22JBZ013）
湖南中医药大学研究生创新项目(2024CX167) （2024CX167）
湖南中医药大学本科生科研创新基金项目(2024BKS124). （2024BKS124）
