湖南中医药大学学报2025，Vol.45Issue(5)：869-876,8.DOI:10.3969/j.issn.1674-070X.2025.05.012
机械敏感性离子通道Piezo2在激痛点炎症诱导机械痛觉过敏中的潜在作用
The potential role of mechanosensitive Piezo2 ion channels in myofascial trigger point inflammation-induced mechanical hyperalgesia
摘要
Abstract
Objective To observe the effects of Piezo2 on mechanical hyperalgesia induced by myofascial trigger point inflammation.Methods Thirty SPF-grade male SD rats were randomly divided into blank group,model group,complete Freund's adjuvant(CFA)group,etoricoxib group,and Piezo2 inhibitor group(hereinafter referred to as the inhibitor group),with six rats in each group.Except for the blank group,models were established in the other four groups at the modeling site of the left vastus medialis muscle.After modeling,the rats in the CFA group received a single 150 uL CFA injection at the trigger point.The etoricoxib group was administered 5.5 mg/kg etoricoxib suspension via gavage twice daily for two consecutive days.The inhibitor group received a single 120 μL injection of Piezo2 inhibitor(60 μg/μL)at the trigger point tissue.Mechanical pain threshold and soft tissue tension were measured before and after intervention.After two weeks of intervention,trigger point samples were collected for microscopic structural observation by HE staining,measurement of interleukin-1 β(IL-1β)levels via ELISA,and analysis of Piezo2 expression using both immunohistochemistry and Western blot.Results Compared with before intervention,the mechanical pain threshold and D0.2kg value in the CFA group decreased after intervention(P＜0.05,P＜0.01),while those in the etoricoxib group and the inhibitor group increased(P＜0.05,P＜0.01).After intervention,compared with the blank group,the model group showed a decrease in mechanical pain threshold and D0.2kg value(P＜0.01);compared with the model group,the mechanical pain threshold and D0.2kg value in the CFA group decreased(P＜0.01),while those in the etoricoxib group and the inhibitor group increased(P＜0.01);compared with the CFA group,the mechanical pain threshold and D0.2kg value increased in both the etoricoxib group and the inhibitor group(P＜0.01).HE staining showed that muscle cells were damaged in the model group and CFA group,with local inflammatory cell infiltration;muscle cell damage was significantly alleviated and local inflammatory cells decreased in both the etoricoxib group and the inhibitor group.Compared with the blank group,the IL-1 β content in the model group increased(P＜0.01);compared with the model group,the content of IL-1β increased in the CFA group(P＜0.01),while those in the etoricoxib group and the inhibitor group decreased(P＜0.01);compared with the CFA group,the levels of IL-1 β decreased in both the etoricoxib group and the inhibitor group(P＜0.01).Compared with the blank group,the model group showed an increase in Piezo2 expression(P＜0.01);compared with the model group,the expression of Piezo2 decreased in both the etoricoxib group and the inhibitor group(P＜0.05),and there was no statistically significant difference in the CFA group(P＞0.05);Compared with the CFA group,the expression of Piezo2 decreased in both the etoricoxib group and the inhibitor group(P＜0.05,P＜0.01).Conclusion(1)CFA can enhance local inflammatory reaction and muscle cell damage in MTrPs,while etoricoxib and the Piezo2 inhibitor can inhibit the inflammatory reaction.(2)Piezo2 may play a role in mechanical hyperalgesia resulted from inflammation in MTrP tissue.关键词
激痛点/Piezo2/炎症反应/外周敏化/机械痛觉过敏/弗氏完全佐剂/依托考昔Key words
myofascial trigger point/Piezo2/inflammatory reaction/peripheral sensitization/mechanical hyperalgesia/Fre-und's complete adjuvant/etoricoxib分类
医药卫生引用本文复制引用
潘杰灵,唐丽亚,张玉乔,闪逸仙,李武,李江山..机械敏感性离子通道Piezo2在激痛点炎症诱导机械痛觉过敏中的潜在作用[J].湖南中医药大学学报,2025,45(5):869-876,8.基金项目
国家自然科学基金面上项目(82174526,82274676,82374613,82474669) （82174526,82274676,82374613,82474669）
湖南省科技创新计划资助项目(2022RC1221). （2022RC1221）
