河北农业科学2025，Vol.29Issue(1)：1-6,6.DOI:10.12148/hbnykx.20250040
谷瘟拮抗木霉筛选与鉴定及其防效研究
Screening,Identification and Control Effect of Trichoderma against Pyricularia grisea
摘要
Abstract
Pyricularia grisea is a major disease in millet production,severely affecting the yield and quality of millet.The use of biocontrol microorganisms to prevent and control P.grisea is the development trend of green plant protection in the future.Using the rhizosphere soil of millet at Shijiazhuang Qiema Experimental Station of the Millet Research Institute of Hebei Academy of Agriculture and Forestry Sciences as the experimental mate-rial,Trichoderma strains were isolated using Rose Bengal medium.The optimal strains were screened using the plate confrontation method,and the strains were identified through a combination of morphological observation and Tef1 sequence analysis.The efficacy of the strains against P.grisea was determined through pot experiments.The results showed that a total of 24 strains with typical characteristics of Trichoderma were screened from the rhizosphere soil samples of millet.Among them,KE-1 strain had the best antagonistic effect,with the inhibition rates of 42.86％,44.44％,57.89％,53.33％,60.00％,and 42.22％against P.grisea,Setosphaeria turcica,Bipolaris maydis,Fusarium graminearum,F.verticil-lioides and Botrytis cinerea,respectively.Based on the comprehensive morphological characteristics and Tef1 sequence analysis results,KE-1 strain was ulti-mately determined to be Trichoderma harzianum.The results of the pot experiment showed that after root irrigation with T.harzianum suspension,the disease severity of millet was significantly reduced,with a control efficacy of 55.56％against the pathogen of P.grisea.In conclusion,T.harzianum strain KE-1 has good biocontrol potential and holds potential application value in the biological control of P.grisea.关键词
哈茨木霉/生防菌/谷子/谷瘟/防治效果Key words
Trichoderma harzianum/Biocontrol bacteria/Millet/Pyricularia grisea/Control effect分类
农业科技引用本文复制引用
王赛雅,刘佳,白辉,连浩然,张梦雅,李志勇,郝志敏..谷瘟拮抗木霉筛选与鉴定及其防效研究[J].河北农业科学,2025,29(1):1-6,6.基金项目
国家现代农业产业技术体系专项(CARS-06-14.5-A25) （CARS-06-14.5-A25）
河北省农林科学院基本科研业务费试点经费包干制项目(HBNKY-BGZ-02) （HBNKY-BGZ-02）
