吉林大学学报（理学版）2025，Vol.63Issue(3)：954-962,9.DOI:10.13413/j.cnki.jdxblxb.2024220
林蛙残体多肽螯合硒的制备及其抗炎活性
Preparation and Anti-inflammatory Activity of Selenium Chelated by Rana dybowskii Residues Peptides
摘要
Abstract
Rana dybowskii residue was used as raw material,the Rana dybowskii residue peptides(RDRP)was abtained under enzymatic hydrolysis conditions,and then were chelated with selenium ions(Se4+)to prepare Rana dybowskii residue peptide chelated selenium(RDRPCS).We characterized the synthesized compounds,studied the optimized conditions for chelation reaction,and investigated their anti-inflammatory activity through in vitro cell models.The experimental results show that Se4+reacts with RDRP to form chelates.RDRPCS has sufficient metal binding sites,with chelating sites being amino,hydroxyl,and C ＝ O.The optimization result of chelation process is that temperature is 60 ℃,pH=6,time is 65 min,and a mass ratio is 2.5∶1,the efficiency of chelation reaction under this conditions is 70.7％.Different concentrations of chelates have inhibitory effects on the release of NO,TNF-α,IL-6 and IL-1β in RAW264.7 inflammatory cell model.关键词
林蛙残体肽/螯合硒/抗炎/细胞模型Key words
Rana dybowskii residues peptides/chelating selenium/anti-inflammatory/cell model分类
生物学引用本文复制引用
徐萍,王君淑,鞠凤霞,李深,张凤清..林蛙残体多肽螯合硒的制备及其抗炎活性[J].吉林大学学报（理学版）,2025,63(3):954-962,9.基金项目
吉林省科技厅重点项目(批准号:20220202073NC). （批准号:20220202073NC）
