国际老年医学杂志2025，Vol.46Issue(3)：296-302,7.DOI:10.3969/j.issn.1674-7593.2025.03.007
基于SIRT1-NF-κB/PGC-1α-BDNF信号通路探讨跑台运动和饮食控制对ApoE-/-小鼠模型认知功能的影响
The effects of treadmill exercise and diet control on cognitive function in HFD-induced ApoE-/-mice based on the SIRT1-NF-κB/PGC-1α-BDNF signaling pathways
摘要
Abstract
Objective To investigate the effect and mechanism of treadmill exercise and diet control on cognitive dysfunc-tion in apolipoprotein E knockout(ApoE-/-)mice model.Methods Forty ApoE-/-male mice fed a high-fat diet for 8 weeks were randomly and equally divided into a high-fat diet exercise(HFD+TR)group,a high-fat diet rest(HFD+SED)group,a diet-con-trolled exercise(DC+TR)group,and a diet-controlled rest(DC+SED)group,with 10 mice in each group.HFD+TR group and HFD+SED group continued to be fed with high-fat diet for 10 weeks,DC+TR group and DC+SED group switched to standard diet for 10 weeks,and the food intake was controlled to 70％of the normal intake,while treadmill exercise intervention was performed in HFD+TR group and DC+TR group.Morris water maze test was used to evaluate the cognitive function of mice,enzyme-linked im-munosorbent assay(ELISA)was used to detect the serum levels of tumor necrosis factor-α(TNF-α)and interleukin-1β(IL-1 β),and real-time PCR(RT-qPCR)was used to detect the expression levels of silent information regulator 1(SIRT1),nuclear transcription factor NF-κB(NF-κB),peroxisome proliferation receptor gamma coactivator α(PGC-1α),and brain-derived neurotrophic factor(BDNF)in the hippocampus.Results The results of water maze test showed that diet control and treadmill exercise could improve spatial learning and memory ability of mice.The results of ELISA and RT-qPCR showed that diet control and treadmill exercise could decrease the levels of inflammatory cytokines TNF-α and IL-1β in the serum of mice,and also decrease the expression levels of NF-κB mRNA in the hippocampus of mice,but increase the expression levels of SIRT1,PGC-1α,and BDNF mRNA in the hippocampus of mice.Conclusion Treadmill exercise and diet control can reduce the inflammatory response in the hippocampus of mice by mediating the SIRT1-NF-κB/PGC-1α-BDNF signaling pathway,thereby improving cognitive function in mice.关键词
运动/饮食控制/高脂饮食/SIRT1-NF-κB/PGC-1α-BDNF/认知功能Key words
Exercise/Diet control/High-fat diet/SIRT1-NF-κB/PGC-1α-BDNF/Cognitive function引用本文复制引用
许婷,邓文娟,李树峰,刘艺,石芷僮,张潇月,王宁萍..基于SIRT1-NF-κB/PGC-1α-BDNF信号通路探讨跑台运动和饮食控制对ApoE-/-小鼠模型认知功能的影响[J].国际老年医学杂志,2025,46(3):296-302,7.基金项目
山西省高等学校科技创新计划项目(2023L480) （2023L480）
山西医科大学汾阳学院科技扶持基金(2022C18) （2022C18）
